UASB处理低磷啤酒废水的微生物群落特性

Microbial Community Characteristics of UASB for Low-phosphorus Brewery Wastewater Treatment

为了探究低磷啤酒废水厌氧处理阶段的微生物种类及群落多样性,将本溪某啤酒厂的低磷特种废水处理工艺中获取的厌氧污泥接种于实验室UASB反应器中,对污泥进行培养驯化直至运行稳定,对低磷厌氧污泥中的细菌和古细菌进行高通量测序分析。UASB系统从启动到稳定历时60 d左右,对COD的去除率在70%以上;系统中污泥的细菌优势菌属为Chlorobium、Longilinea、Methanothrix、Levilinea等,古细菌优势菌属为Methanothrix、Methanobacterium。与常规厌氧污泥相比,低磷啤酒废水处理系统的厌氧污泥中硫化细菌和硫酸盐还原菌的含量较高,为优势菌属。

To investigate the microbial community and diversity in anaerobic treatment of low-phosphorus brewery wastewater,the anaerobic sludge obtained from a special low-phosphorus wastewater treatment process of a brewery in Benxi was inoculated in a laboratory-scale UASB,and the bacterial and archaeal communities of the cultivated anaerobic sludge for low-phosphorus wastewater treatment were analyzed by high-throughput sequencing.It took approximately 60 days for the UASB system from start-up to stabilization,and the COD removal efficiency was above 70%.The dominant bacterial genera of the sludge in the system were Chlorobium,Longilinea,Methanothrix,Levilinea,etc.,and the dominant archaea were Methanothrix and Methanobacterium.Compared with other conventional anaerobic sludge,the relative abundance of sulfur-oxidizing bacteria and sulfate-reducing bacteria in the anaerobic sludge for low-phosphorus brewery wastewater treatment was higher,which were the dominant bacteria.