补充维生素D对中年雄性小鼠精子活力的影响

Effects of vitamin D supplementation on sperm motility in middle aged male mice

目的探讨补充维生素D(vitamin D,VD)对中年雄性小鼠精子活力的影响。方法采用VD常规含量饲料喂养的7周龄雄性ICR小鼠随机分为4组:中年VD缺乏组、中年常规VD组、中年补充VD组、年轻常规VD组(每组7只),前3组小鼠8~28周龄分别给予VD缺乏、常规含量、高于常规含量的饲料(VD含量分别为<100、1000及1500 U/kg饲料)喂养,后一组小鼠持续采用VD常规含量的饲料喂养至8周龄。利用酶联免疫吸附测定(ELISA)法测定血清25-羟维生素D(25OHD)水平,采用计算机辅助精液分析系统检测精液各项参数。结果中年补充VD组小鼠的血清25OHD水平显著高于其他3组小鼠,中年常规VD组和年轻常规VD组的血清25OHD水平显著高于中年VD缺乏组。各组间小鼠的睾丸和附睾的平均重量未见显著性差异。与中年常规VD组、中年VD缺乏组相比,中年补充VD组小鼠的精子活动率和前向运动速度显著升高,达到接近年轻常规VD组小鼠的水平。结论补充VD有助于维持中年雄性小鼠的精子活力。

Objective To investigate the effect of vitamin D(VD)supplement on sperm motility in middle aged male mice.Methods Twenty-eight male ICR mice fed with regular VD diet aged 7 weeks,were randomized into four groups:middle aged VD-deficiency group,middle aged VD-regular group,middle aged VD-supplement group,and younger VD-regular group(n=7 in each group).Mice in the first three groups were fed with very low VD diet<100 U/kg,regular VD diet 1000 U/kg,and high VD diet 1500 U/kg,respectively from week 8th to week 28th,and the mice in younger VD-regular group were fed with regular VD diet up to week 8th.The level of 25-hydroxyvitamin D(25OHD)was determined by ELISA,and the semen parameters were determined by computer aided semen analysis system.Results The serum 25OHD level in the middle aged VD-supplement group was higher than that in the other three groups,and the levels of serum 25OHD in the middle aged and younger VD-regular groups were higher than that in the middle aged VD-deficiency group.There were no significant differences in the average weight of testis and epididymis among all groups.Compared with the middle aged VD-regular group and the middle aged VD-deficiency group,the sperm motility rate and straight-line velocity in the middle aged VD-supplement group were significantly elevated,approximate to the levels in younger VD-regular group.Conclusion VD supplement is helpful to maintain the sperm motility in middle aged male mice.