青娥方对膝骨关节炎伴骨质疏松模型大鼠早期软骨下骨整合素及抑癌基因表达的影响

Efficacy of Qing'e Decoction on the Expression ofαvβ3 Integrin and P53 Gene in Subchondral Bone of Knee Osteoarthritis with Osteoporosis Model Rats

目的:观察青娥方药液对膝骨关节炎伴骨质疏松模型大鼠软骨下骨中整合素αvβ3及P53基因表达的影响。方法:将30只SD大鼠编号后,随机分为正常组(A组)、青娥方组(B组)、骨质疏松组(C组)、骨关节炎伴骨质疏松组(D组)和骨关节炎组(E组),每组6只。将B组、C组和D组大鼠腹腔注射麻醉后,行骨质疏松造模。1个月后,再将B组、D组、E组三组大鼠麻醉后行骨关节炎造模,术后行相同处理。经二次造模1d后,B组大鼠持续4周每天灌服1次药液。其余各组大鼠以相同剂量每天灌服1次生理盐水。结果:正常组整合素αvβ3基因在第2,4周均呈弱上调表达,表达水平维持相对稳定;B组整合素αvβ3基因表达随着时间的推移先呈现出较A组增高的水平,但逐渐出现下降趋势;B组整合素αvβ3及P53基因水平表达相对稳定,趋近A组;相较于D组,B组大鼠经青娥方治疗后,其胫骨髁软骨下骨组织中的整合素αvβ3基因表达水平在第2,4周的差异有统计学意义(P<0.05)。C组、D组、E组三个时间点P53基因均呈弱上调表达,随着时间的推移,P53表达水平小幅度上升;B组P53基因表达水平与A组差异无统计学意义(P>0.05),且低于同时期其他三组。结论:青娥方能够抑制整合素αvβ3在软骨下骨组织中的表达,从而降低破骨细胞活性,减少骨吸收,有利于促进骨吸收、骨形成平衡的恢复,而对P53基因表达的抑制作用尚不明显。

Objective:To observe the efficacy of Qing'e prescription onαvβ3integrin and P53gene expression levels in subchondral bone tissue of osteoporotic knee osteoarthritis(KOA)rats,and to explore the mechanism of Qing'e policy on subchondral bone tissue in further discussion,so as to provide theoretical reference for the clinical treatment of osteoporotic KOA.Methods:30SD rats were randomly divided into normal group(group A),Qing'e prescription group(group B),osteoporosis model group(group C),osteoporosis osteoarthritis model group(group D)and osteoarthritis model(group E),with 6rats in each group.Their osteoporosis models were established after group B,group C and group D rats being anesthetized by intraperitoneal injection.The osteoarthritis model of rats in groups B,D and E was established after anesthesia,and the same treatment was performed after operation one month after modeling.Then group B was perfused with Qing'e prescription solution on the second day after operation,and the other groups were perfused with the same amount of normal saline,once a day for 4weeks.Results:The expression ofαvβ3integrin was weakly up-regulated at 2and 4weeks in group A,and the expression level remained relatively.With the passage of time,theαvβ3integrin expression in group B first increased compared with group A,but gradually decree sed stable.Compared with group D,the difference of theαvβ3integrin expression level in the subchondral bone tissue of tibial condyle in group B after treatment with Qing'e prescription in the second and fourth weeks was statistically significant.The expression of P53gene was weakly up-regulated in group C,group D and group E.With the passage of time,the expression level of P53increased slightly,there was no significant difference in P53gene expression between group B and group A,and it was lower than that of the other three groups at the same time.Conclusion:Qing'e prescription can inhibit the expression ofαvβ3integrin in subchondral bone tissue,and reduce the bone resorption of subchondral bone in osteoporotic osteoarthritis,and inhibit the imbalance of bone resorption in the process of bone reconstruction,but the inhibitory efficacy on P53gene expression is not obvious.