叶酸氮共掺杂石墨烯量子点复合物对M1型巨噬细胞叶酸受体β的靶向作用

Targeting effect of folic acid nitrogen-doped graphene quantum dot complex on folate receptor 2 of M1 macrophages

目的研究叶酸氮共掺杂石墨烯量子点(folic acid nitrogen-doped graphene quantum dots,FA-N-GQDs)复合物对M1型巨噬细胞叶酸受体β(folate receptor 2,FOLR2)的靶向作用。方法用佛波酯(phorbol12-myristate13-acetate,PMA)将THP-1细胞诱导成M0型巨噬细胞,再分别使用脂多糖(LPS)+干扰素γ(IFNγ)和白细胞介素-4(IL-4)+IL-13将M0型巨噬细胞诱导成M1和M2型巨噬细胞;将FA-N-GQDs复合物分别与M1和M2型巨噬细胞共培养,RT-PCR及Western blot法检测细胞表面FOLR2 mRNA及蛋白水平;荧光法观察FOLR2的靶向情况。结果M1型巨噬细胞FOLR2表达量高于M0和M2型巨噬细胞;荧光显微镜下,仅M1型巨噬细胞与FA-N-GQDs复合物共培养组可见明显荧光,其他组均未见明显荧光。结论FA-N-GQDs复合物对M1型巨噬细胞上的FOLR2具有较好的靶向作用。

Objective To investigate the targeting effect of folic acid nitrogen-doped graphene quantum dot(FA-N-GQD)complex on folate receptor 2(FOLR2)of M1 macrophages.Methods THP-1 cells were induced with phorbol12-myristate13-acetate(PMA)into M0 macrophages which were further induced with lipopolysaccharide(LPS)+interferonγ(IFNγ)and interleukin-4(IL-4)+IL-13 into M1 and M2 macrophages respectively.FA-N-GQD complex was co-cultured with M1 and M2 cells respectively.The expression of FOLR2 at mRNA and protein levels were determined by RT-PCR and Western blot respectively,while the FOLR2 targeting was observed by fluorescence assay.Results The FOLR2expression levels in M1 macrophages was higher than those in M0 and M2 microphages.Obvious fluorescence was observed only in M1 macrophages co-incubated with FA-N-GQD complex but not in other groups under fluorescent microscope.Conclusion FA-N-GQD complex showed good targeting effect on FOLR2 of M1 macrophages.