2个大豆VOZ转录因子的克隆及非生物胁迫表达分析

Clone and expression analysis of two VOZ transcription factors in soybean under abiotic stress

为探究大豆维管植物锌指蛋白(VOZ)转录因子的序列特征和非生物胁迫表达模式,采用实时荧光定量PCR对Gm VOZ1-1,Gm VOZ1-2在非生物胁迫下的表达量进行检测,并对其进行克隆及序列分析.Gm VOZ1-1,Gm VOZ1-2对干旱、高盐、低温胁迫均存在响应,且干旱胁迫下表达量升高最明显.Gm VOZ1-1位于大豆6号染色体上,Gm VOZ1-2位于大豆13号染色体上,分别编码含有478,459个氨基酸的蛋白质,它们均含有1个保守的VOZ结构域.Gm VOZ1-1,Gm VOZ1-2均含有1个核定位信号,预测均为细胞核蛋白.Gm VOZ1-1与白羽扇豆La VOZ的亲缘关系最近,GmVOZ1-2与蒺藜苜蓿Mt VOZ的亲缘关系最近.

In order to investigate the sequence characteristics of VOZ transcription factors in soybean and their expression patterns under abiotic stress,the expression levels of Gm VOZ1-1,Gm VOZ1-2 under abiotic stress were detected by real-time fluorescence quantitative PCR,and the gene cloning and sequence analysis were also carried out.Gm VOZ1-1,Gm VOZ1-2 responded to drought,salt and cold stresses,and their expression levels increased most obviously under drought stress.Gm VOZ1-1 was located on chromosome 6 of soybean and encoded a protein containing 478 amino acids.Gm VOZ1-2 was located on chromosome 13 of soybean and encoded a protein containing 459 amino acids.The amino acid sequences of GmVOZ1-1,GmVOZ1-2 both contained a conserved VOZ domain.GmVOZ1-1 and Gm VOZ1-2 both contained a nuclear localization signal,which were predicted to be nuclear proteins.GmVOZ1-1 was most closely related to LaVOZ of Lupinus albus,and GmVOZ1-2 was most closely related to MtVOZ of Medicago truncatula.