摘要
Since the anti-inflammatory effect of hydrogen has been widely known,it was supposed that hydrogen could suppress tissue damage by inhibiting virus-related inflammatory reactions.However,hydrogen is slightly soluble in water,which leads to poor effect of oral hydrogen-rich water therapy.In this study,the nano-bubble hydrogen water(nano-HW)(about 0.7 ppm)was prepared and its therapeutic effect against viral infection was investigated by utilizing spring viraemia of carp virus(SVCV)-infected zebrafish as model.Three-month-old zebrafish were divided into nano-HW treatment-treated group and aquaculture water treated group(control group).The results revealed that the cumulative mortality rate of SVCV-infected zebrafish was reduced by 40%after treatment with nano-bubble hydrogen water,and q RT-PCR results showed that SVCV replication was significantly inhibited.Histopathological examination staining showed that SVCV infection caused tissue damage was greatly alleviated after treatment with nano-bubble hydrogen water.Futhermore,SVCV infection caused reactive oxygen species(ROS)accumulation was significantly reduced upon nano-HW treatment.The level of proinflammatory cytokines IL-1β,IL-8,and TNF-αwas remarkably reduced in the nano-HW-treated group in vivo and in vitro.Taken together,our data demonstrated for the first time that nano-HW could inhibit the inflammatory response caused by viral infection in zebrafish,which suggests that nano-HW can be applied to antiviral research,and provides a novel therapeutic strategy for virus-caused inflammation related disease.
基金
supported by Natural Science Foundation of China(31972834,32022082,31972721)。
