葡萄汁有孢汉逊酵母BF-345的高密度培养研究

Study on High Cell-density Culture of Hanseniaspora uvarum BF-345

为研究一株本土葡萄汁有孢汉逊酵母(Hanseniaspora uvarum,H.uvarum)菌株BF-345高密度生长的培养基组分,采用单因素试验结合响应面法对培养基中的碳源、氮源、无机盐等成分进行优化,以生长量进行表征。结果表明,以葡萄糖34.683 g/L、酵母浸粉4.178 g/L、牛肉膏18.533 g/L、磷酸二氢钾5.683 g/L为培养基组分,在28℃,180 r/min条件下,摇床震荡培养18 h,菌株BF-345活菌数可达到5.673×10^(9)CFU/mL,比优化前高出一个数量级。研究结果表明葡萄糖更适用于H.uvarum菌株BF-345的吸收与利用,酵母浸粉与牛肉膏复合使用对其增殖作用显著优于单一氮源,且维生素B1、维生素B2、肌醇、烟酰胺作为生长因子不适用于其增殖培养。

To study the medium components for the high-density growth of a native H.uvarum BF-345.The single factor experiment combined with the response surface method was used to optimize the carbon source,nitrogen source,inorganic salt and other components in the medium,and the growth was characterized.The optimized medium method yielded the following results:glucose 34.683 g/L,yeast extract 4.178 g/L,beef extract 18.533 g/L,and KH_(2)PO_(4)5.683 g/L,the culture was incubated at 28℃and 180 r/min with shaking in a shaker.When H.uvarum was cultured under these conditions for 18 hours,it achieved a viable count of 5.673×10^(9)CFU/mL,an order of magnitude higher than before optimization.Furthermore,glucose was better suited for H.uvarum absorption and utilization.The effect of yeast extract combined with beef extract on H.uvarum proliferation was significantly greater than that of a single nitrogen source,vitamins(B1 and B2),inositol,and nicotinamide,indicating that these growth factors were insufficient for yeast proliferation.