两种新型荧光黏度探针的合成及光学性质研究

Synthesis and optical properties of two novel fluorescent viscosity probes

以吲哚-3-甲醛、2-甲基苯并噻唑和2-甲基喹啉为原料,通过缩合反应合成了两种D-π-A构型的黏度荧光探针(E)-3-(2-(2-(1-丁基-苯并吲哚-3-烃基)乙烯基)苯并噻唑-3-烃基)丙烷-1-磺酸盐(L1)和(E)-3-(2-(2-(1-丁基-苯并吲哚-3-烃基)乙烯基)喹啉-1-烃基)丙烷-1-磺酸盐(L2).运用荧光光谱法研究了探针对黏度的响应性质及其他分析物对探针的干扰性质.由于探针具有较好的生物相容性,可望用于人肝癌细胞(HepG2)内微环境黏度的荧光成像.

Two D-π-A type fluorescent viscosity probes(E)-3-(2-(2-(1-butyl-1 H-indol-3-yl)vinyl)benzo[d]thiazol-3-ium-3-yl)propane-1-sulfonate(L1) and(E)-3-(2-(2-(1-butyl-1 H-indol-3-yl)vinyl)quinolin-1-ium-1-yl)propane-1-sulfonate(L2) were synthesized from indole-3-formaldehyde, 2-methylbenzothiazole and 2-methylquinoline by condensation reaction.The response properties of these probes to viscosity and the interference properties of other analytes to the probe were investigated by fluorescence spectroscopy.Given the good biocompatibility, these probes can be used for fluorescence imaging of microenvironmental viscosity in hcuman hepatoellular carcinomas(HepG2) cells.