正交实验法优选升清祛湿汤提取工艺的研究

Study on Optimized Extraction Process of Shengqing Qushi Decoction by Orthogonal Experiment

目的:利用正交实验法建立升清祛湿汤的最佳提取工艺。方法:以葛根素为检测指标,利用正交实验法从煎煮次数、煎煮时间、加水量等维度考察升清祛湿汤提取的最佳条件。结果:升清祛湿汤的最佳提取工艺为加水煎煮2次、加水量为8倍、单次煎煮时间为1 h,即第1次加8倍量水、煎煮1 h,第2次加8倍量水、煎煮1 h。结论:优化后升清祛湿汤的提取工艺可以提高提取效率,节省制备时间,从而更好地满足临床的需求。

Objective:To establish an optimal extraction process of Shengqing Qushi decoction by orthogonal experiment method.Methods:The orthogonal experiment method was used to investigate the optimal extraction conditions for Shengqing Qushi decoction in terms of frequency of decoction,decoction time,amount of added water,etc.by using puerarin as a detection index.Results:The optimal extraction process of Shengqing Qushi decoction was as follows:decocting twice by adding 8-fold of water for 1 hour each time,namely,decocting for the first time by adding 8-fold of water for 1 hour,and decocting for the second time by adding 8-fold of water for 1 hour.Conclusion:The optimized extraction process of Shengqing Qushi decoction can improve the extraction efficiency and save the preparation time so as to better satisfy the clinical needs.