早期动脉粥样硬化中炎症细胞的分布情况

Distribution of inflammatory cells in early atherosclerosis

目的分析早期动脉粥样硬化小鼠炎性微环境中免疫细胞组成情况。方法高脂饮食喂养ApoE^(-/-)小鼠构建早期动脉粥样硬化模型,以普通饲料喂养的C57小鼠作对照;运用油红O染色、Masson染色及生化分析仪评价模型构建是否成功;流式细胞术检测并分析小鼠主动脉、外周血、脾脏及骨髓中固有免疫细胞和适应性免疫细胞浸润情况。结果与C57小鼠相比,ApoE^(-/-)小鼠主动脉根部脂质浸润增加、血脂水平明显升高(P<0.01);ApoE^(-/-)小鼠主动脉和外周血中性粒细胞、肥大细胞、自然杀伤细胞和单核/巨噬细胞比例较C57小鼠明显增加(P<0.05),且浸润的单核/巨噬细胞以M1型为主(P<0.05);ApoE^(-/-)小鼠主动脉、外周血和脾脏中浸润的CD3+T细胞、Th17细胞及Tc17细胞的比例明显升高(P<0.05)或有升高趋势。结论在动脉粥样硬化早期,多种固有免疫细胞和适应性免疫细胞促进了其炎性微环境的形成,在动脉粥样硬化的形成中发挥重要作用。

Aim To analyze the composition of immune cells in the inflammatory microenvironment of early atherosclerotic mice.Methods ApoE^(-/-) mice fed with high fat diet were used to establish early atherosclerotic mouse model,and C57BL/6 mice fed normal diet were used as control.Oil red O staining,Masson′s staining and biochemical analyzer were used to evaluate the success of the model construction.Infiltration of innate immune cells and adaptive immune cells in aorta,peripheral blood,spleen and bone marrow of mice was detected and analyzed by flow cytometry.Results Compared with C57 mice,lipids infiltration in aortic root of ApoE^(-/-) mice increased and lipids level significantly increased(P<0.01).Compared with those of C57 mice,the proportions of neutrophils,mast cells,natural killer cells and monocytes/macrophages in the aorta and peripheral blood of ApoE^(-/-) mice significantly increased(P<0.05)and the infiltrated monocytes/macrophages were mainly pro-inflammatory M1 monocytes/macrophages(P<0.05).The percentages of infiltrating CD3+T cells,Th17 cells and Tc17 cells in the aorta,peripheral blood and spleen of ApoE^(-/-) mice significantly increased(P<0.05)or had an increasing tendency.Conclusions At the early stage of atherosclerosis,a variety of innate immune cells and adaptive immune cells promote the formation of inflammatory microenvironment,which plays an important role in the initiation of atherosclerosis.