中国南瓜蔗糖转化酶(INV)基因的鉴定及进化和表达分析

Analysis on identification,evolution and expression of sucrose invertase gene(INV)in Cucurbita moschata

蔗糖转化酶(INV)编码基因广泛参与植物的生长发育,在果实成熟期可影响果实品质。本研究利用生物信息学手段对中国南瓜INV基因家族进行鉴定和分析。结果表明,从中国南瓜中鉴定出18个INV基因家族成员,其功能分为酸性转化酶和中性/碱性转化酶两大类。该家族成员在11条染色体上不均匀分布,且仅有1对串联重复基因。表达模式分析结果显示,CmoCh06G004890.1在根中的相对表达量高于其他INV基因家族成员,CmoCh01G010160.1和CmoCh06G004890.1在果实中的相对表达量较高。葫芦科作物INV系统进化分析结果表明,中国南瓜、印度南瓜和美洲南瓜的大多数INV基因均聚为一簇,具有较近的亲缘关系。本研究结果为进一步分析中国南瓜INV基因的生物学功能以及提高中国南瓜品质提供了一定的理论依据。

Invertase(INV)encoding genes are widely involved in plant growth and development,and can affect fruit quality during ripening stage.In this study,INV gene family of Cucurbita moschata was identified and analyzed by bioinformatic methods.The results showed that,18 members of INV gene family were identified from C.moschata,and their corresponding functions were encoding acid INV and neutral/alkaline INV.The members of INV gene family distributed unevenly on 11 chromosomes and there only existed one pair of tandem duplicated genes.Analysis of expression pattern showed that,the relative expression level of CmoCh06G004890.1 in roots of C.moschata was higher than other members of INV gene family.The relative expression levels of CmoCh01G010160.1 and CmoCh06G004890.1 were relatively high in fruits of C.moschata.Results of phylogenetic analysis of INV genes in Cucurbitaceae crops showed that,Most of the INV genes from C.moschata,C.maxima and C.pepo gathered into one cluster and had close genetic relationships.The results can provide theoretical basis for further analyzing of the biological functions of INV genes and improving fruit quality of C.moschata.