青霉MGC07产1,5-α-L-阿拉伯聚糖酶的调控研究

Regulation of 1,5-α-L-Arabanase Produced by Penicillium sp.MGC 07

阿拉伯聚糖的酶解产物L-阿拉伯糖、阿魏酰阿拉伯寡糖、阿拉伯寡糖功能独特,但目前对阿拉伯聚糖水解酶的研究较少,尚未开发出具有商业应用潜力的阿拉伯聚糖酶。该研究从土壤中筛选出一株产1,5-α-L-阿拉伯聚糖酶的真菌,结合产孢结构观察和分子生物学鉴定,确定为一株青霉(Penicillium sp.),命名为Penicillium sp.MGC 07。采用单因素法和响应面法优化该菌发酵产酶的条件,结果表明,该菌在甜菜粕39 g/L、磷酸氢二铵16 g/L、初始pH7、培养温度21℃调控条件下,发酵6 d时达到1,5-α-L-阿拉伯聚糖酶活力高峰,酶活为4.3 U/mL,是优化前的3倍。该菌株较优良的产酶能力可为该酶以酶法制备阿拉伯寡糖和阿魏酰阿拉伯寡糖提供理论支持。

The enzymatic hydrolysis products of arabinan,L-arabinose,feruloyl arabino-oligosaccharide,and arabino-oligosaccharide,have unique functions and great application potential.However,there are few studies on arabinan hydrolase,and arabanase commercial application potential hasn’t been developed yet.I n this study,a 1,5-α-L-arabanase-producing f ungus was screened f rom the soil.C ombined with the observation of the spore-forming structure and the identification of molecular biology,it was identified as a Penicillium sp.and named as Penicillium sp.MGC 07.The single factor method and response surface methodology were used to optimize the fermentation conditions of the bacterium for enzyme production.The results showed that under the control conditions of beet meal 39 g/L,(NH_(4))_(2)HPO_(4)16 g/L,initial pH 7,and culture temperature 21℃,1,5-α-L-arabanase activity peak was achieved at 6 days after bacterium fermentation,the enzyme activity was 4.3 U/mL,which was 3 times that before optimization.The excellent enzyme-producing ability of this strain can provide theoretical support for the enzymatic preparation of arabino-oligosaccharide and feruloyl-arabino-oligosaccharide.