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缺血性脑卒中患者的肠道微生物菌群多样性研究及相关危险因素分析

Study on intestinal microbiota diversity and related risk factors in patients with ischemic stroke
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摘要 目的 探讨急性缺血性脑卒中患者肠道菌群的多样性和分布变化,为缺血性脑卒中的临床治疗提供新的思路。方法 收集2020年11月至2021年1月于山东省济南市章丘区人民医院就诊的30例急性脑卒中患者和30例体检健康者的一般资料、血清学组织和粪便组织。比较分析两组患者性别、年龄、基础疾病、化验检查等的不同。采用16S测序方法对两组研究对象的粪便标本进行测序分析,比较两组患者肠道菌群生物多样性、物种结构、物种差异等的不同。结果 两组研究对象在基线资料一致,缺血性脑卒中患者高血压患者比例更高,差异具有统计学意义。化验结果分析,缺血性脑卒中患者白细胞、血脂、胆固醇水平明显高于对照组,差异具有统计学意义。16S测序分析显示与对照组患者相比,急性脑卒中患者的肠道菌群多样性减少。与对照患者相比,急性脑缺血组患者普雷沃菌属(Prevotella)、egamonas表达升高,类杆菌属(Bacteroides)表达降低。LEfSe多级物种差异分析结果提示急性脑缺血组的特征菌群为普雷沃氏菌科(Prevotellaceae)和Prevotella,对照患者的特征菌群为梭菌目(Clostridiales)和Bacteroides。结论 缺血性脑卒中患者存在肠道菌群多样性减少、有益菌减少,对脑卒中患者进行肠道菌群干预可能有助于患者疾病的恢复。 Objective To explore the diversity and distribution of gut microbiota in patients with acute cerebral infarction and to provide new insights for the clinical treatment of this kind of disease.Methods The general data,serological tissue and fecal tissue of 30 patients with acute stroke and 30 healthy controls treated in Jinan Zhangqiu District People′s Hospital,Shandong Province from November 2020 to January 2021 were collected.The differences of gender,age,basic diseases and laboratory examinations between the two groups were compared and analyzed.16S sequencing method was used to sequence and analyze the fecal samples of the two groups,and the differences of intestinal flora biodiversity,species structure and species differences between the two groups were compared.Results The baseline data of the two groups were consistent.The proportion of patients with ischemic stroke and hypertension was higher,and the difference was statistically significant.The analysis of test results showed that the levels of leukocyte,blood lipid and cholesterol in patients with ischemic stroke were significantly higher than those in the control group,and the difference was statistically significant.Compared with the control group,the diversity of intestinal flora in patients with acute stroke was reduced.Compared with the control patients,the expression of Prevotella and egamonas increased and the expression of Bacteroides decreased in the acute cerebral ischemia group.The results of lefse multi-level species difference analysis showed that the characteristic flora of acute cerebral ischemia group were prevotellaceae and Prevotella,and the characteristic flora of control patients were clostridiales and Bacteroides.Conclusion The diversity of intestinal flora and beneficial bacteria in patients with ischemic stroke are reduced.The intervention of intestinal flora in patients with stroke may be helpful to their recovery.
作者 郭琳琳 李冰清 GUO Linlin;Li Bingqing(Department of Pathogen Biology,School of Basic Medicine,Shandong First Medical University,Jinan,Shandong 250200,China;Department of Neurology,Jinan Zhangqiu District People's Hospital,Jinan,Shandong 250200,China)
出处 《国际检验医学杂志》 CAS 2022年第S01期1-5,共5页 International Journal of Laboratory Medicine
基金 国家自然科学基金(32170034)。
关键词 急性缺血性脑卒中 肠道菌群 多样性 危险因素 acute ischemic stroke intestinal flora diversity risk factors
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