当归抽薹关键基因FT和SOC1的生物信息学分析

Bioinformatics Analysis of Key Genes FT and SOC1 in Radix Angelica sinensis

当归是一种药用价值较高的重要的多年生草本大宗药材,但当归抽薹严重影响其产量和品质,这是限制大规模人工种植栽培当归的关键瓶颈。本研究从当归中克隆到FT和SOC1两个候选基因,并通过序列同源比对分析候选基因与当归抽薹的关联性。结果表明,FT基因全长为528 bp,编码175个氨基酸,生物信息学分析显示,FT蛋白属于不稳定蛋白,在膜外进行作用,主要二级结构形式为延伸链和无规卷曲。SOC1基因共654 bp,编码217个氨基酸,生物信息学分析显示,SOC1蛋白属于不稳定蛋白,主要二级结构形式为α-螺旋和无规则卷曲。发育树聚类显示本研究克隆的FT基因编码的氨基酸序列与胡萝卜FT基因编码的氨基酸序列和胡萝卜亚种栽培种‘heading date 3A-like’蛋白的氨基酸序列均有较高同源性。SOC1基因编码的蛋白与胡萝卜亚种栽培种MADS-box基因家族中SOC1类似蛋白有较高的同源性。综上推测本研究中分离出的FT和SOC1两个基因很有可能与当归开花性状密切相关。本研究结果对解析当归抽薹相关分子机制提供理论参考,为当归的改良育种提供分子依据。

Angelica sinensis is an important perennial herb with high medicinal value, but bolting seriously affects its yield and quality, which is a key bottleneck restricting large-scale artificial cultivation of Angelica sinensis. In this study, two candidate genes FT and SOC1 were cloned from Angelica sinensis, and the relationship between candidate genes and Angelica was analyzed by sequence homology comparison. The results showed that the length of the FT gene was 528 bp, encoding 175 amino acids. Bioinformatics analysis shows that FT protein is an unstable protein that acts outside the membrane. The main secondary structure forms are extension cords and random coils.The SOC1 gene is 654 bp in total, encoding 217 amino acids. Bioinformatics analysis shows that SOC1 protein is an unstable protein, and the main secondary structure forms are α-helix and random coil. The clustering of the developmental tree showed that the amino acid sequence encoded by the FT gene cloned in this study has a high degree of homology with the amino acid sequence encoded by the carrot FT gene and the amino acid sequence of the carrot subspecies ’heading stage 3A-like’ protein. The protein encoded by the SOC1 gene has a high degree of homology with the SOC1-like protein in the MADS-box gene family of carrot subspecies. In summary, it is speculated that the two genes FT and SOC1 isolated in this study may be closely related to the flowering characteristics of Angelica sinensis. This research provides a theoretical reference for the analysis of the related molecular mechanism of Angelica, and provides a molecular basis for the improved breeding of Angelica sinensis.