gma-miR4359b抑制载体的构建及表达模式分析

Construction of gma-miR4359b Inhibitory Vector and Analysis of Its Expression Pattern

MicroRNA在植物生长发育、逆境响应等多个方面具有重要的调控作用。相比保守的miRNA,存在于特定物种中的非保守性miRNA研究较少。研究发现非保守miRNA在调控作物经济性状和应对非生物胁迫等方面发挥重要作用。为了研究大豆非保守miRNA gma-miR4359b的生物学意义,本试验通过重叠PCR方法替换拟南芥内源性IPS1基因构建了抑制大豆非保守miRNA gma-miR4359b表达的mimicry4359b载体,采用RT-qPCR方法检测gma-miR4359b在大豆不同组织的相对表达量,结果表明gma-miR4359b在根中表达量最高。本研究结果为进一步研究gma-miR4359b的生物学功能与作用机制提供参考。

MicroRNA plays an important role in plant growth, development, stress response and many others aspects. Non-conserved miRNAs in some species have been less studied than conserved miRNAs. It was found that non-conserved miRNAs play an important role in regulating economic traits of crops and responding to abiotic stress. In order to study the biological significance of non-conserved miRNAs gma-miR4359b in soybean,this study repleced the endogenous IPS1 gene of Arabidopsis thaliana by overlapped PCR to constructe the mimicry4359b vector to inhibit the expression of no-conserved miRNAs gma-miR4359b. The relative expression of gma-miR4359b in different tissues of soybean was detected by RT-qPCR. The results showed that the expression of gma-miR4359b was the highest in the root. This study laid a foundation for further study on the function and mechanism of gma-miR4359b.