福建大头蛙抗癌多肽FJ-2945抗肿瘤活性研究

Research on the antitumor effect of the peptide FJ-2945 from the skin secretion of Fujian large head frog

探索本课题组自主发现的天然抗肿瘤多肽FJ-2945对肝癌细胞Hep3b增殖、迁移的影响并初步明确其作用机制.通过CCK8与RTCA实验检测FJ-2945对细胞增殖能力的影响;采用Transwell与划痕实验分析FJ-2945对于Hep3b迁移能力的影响;利用流式细胞分析法检测FJ-2945对Hep3b细胞周期的影响;最后通过qRT-PCR与免疫印迹实验检测FJ-2945对Hep3b细胞增殖、迁移相关因子在转录水平与蛋白水平的影响.与对照组相比,FJ-2945显著抑制Hep3b细胞的增殖与迁移能力,并促进Hep3b细胞凋亡.实验组中的Skp2蛋白含量显著降低,最终抑制Hep3b细胞增殖与迁移能力.由此说明多肽FJ-2945可以作为潜在的药物,通过靶向Skp2抑制肝癌细胞Hep3b增殖、迁移等过程,发挥抗肿瘤作用.

To explore the anticancer effect of the novel natural peptide FJ-2945 against the proliferation and migration process of human hepatocarcinoma Hep3b,and clarified the related anticancer mechanisms.The effect of FJ-2945 on the proliferation process of Hep3b cells was investigated by CCK8 and RTCA assays.The migration inhibition effect of FJ-2945 was detected by using Transwell and wound assays.The effect of FJ-2945 on the cell cycle distribution of Hep3b cells was investigated by using flow cytometric analysis.qRT-PCR and immunoblotting assay were used to detect the alteration of in transcription and protein levels of the proliferation and migration factors of Hep3b.Compared to the control group,FJ-2945 exhibited a dose-dependent inhibition of the proliferation and migration of Hep3b,while induced the apoptotic process of Hep3b.Protein level of Skp2 was significantly reduced in the FJ-2945 group.The downregulation of Skp2 ultimately interfered the proliferation and migration process of Hep3b.These results suggest that peptide FJ-2945 can be used as a anti-tumour therapy to inhibit the proliferation and migration of Hep3b cells by targeting Skp2.