响应曲面法联合遗传算法优化天然低共熔溶剂提取肉苁蓉中苯乙醇苷类成分的工艺

Optimization of natural deep eutectic solvent extraction of phenylethanoid glycosides from Cistanche deserticola by response surface methodology coupled with genetic algorithm

目的优化天然低共熔溶剂(NADESs)提取肉苁蓉中苯乙醇苷类成分的工艺,为肉苁蓉的开发和利用提供参考。方法首先,以松果菊苷、毛蕊花糖苷和异毛蕊花糖苷的总提取效率为指标,筛选NADESs的类型;然后,在单因素实验基础上,通过响应曲面法优选最佳NADESs组成成分的摩尔比、NADESs含水量和液料比,并通过遗传算法对结果进行优化。以维生素C(VC)为阳性对照,从提取效率、体外抗氧化活性2个方面比较NADESs和传统溶剂(50%甲醇)的提取效果。结果最佳提取溶剂为由1,4-丁二醇和丙二酸组成的NADES-11。最优提取工艺为:NADES-11中1,4-丁二醇和丙二酸的摩尔比1∶2.5、NADES-11含水量18%、液料比30 mL/g、提取时间30 min、提取温度30℃。NADES-11的提取效率显著高于50%甲醇(P<0.05);NADES-11提取物对1,1-二苯基-2-三硝基苯肼自由基和羟基自由基的半数清除浓度(261.17、744.34μg/mL)均低于50%甲醇提取物(420.97、1175.12μg/mL),其抗坏血酸当量(17.19、360.80 mg VC/g)高于50%甲醇提取物(10.67、228.54 mg VC/g)。结论本研究优化的NADESs提取肉苁蓉中苯乙醇苷类成分的工艺环保、稳定、可行。

OBJECTIVE To optimize the extraction technology for phenylethanol glycosides from Cistanche deserticola by natural deep eutectic solvents(NADESs),and to provide reference for the development and utilization of C.deserticola.METHODS The optimal NADESs was selected using total extraction efficiency of echinacoside,acteoside and isoacteoside as indexes.Based on single factor test,response surface methodology was used to select the optimal NADESs molar ratio,the optimal NADESs water content,the optimal liquid-solid ratio;and the results were optimized by genetic algorithm.Using vitamin C(VC)as positive control,the extraction effects of NADESs and traditional solvent(50%methanol)were compared in respects of extraction efficiency and antioxidant activities.RESULTS The optimal extraction solution was NADES-11 composed of 1,4-butanediol and malonic acid.The optimal extraction technology was as follows as the molar ratio of 1,4-butanediol-malonic acid was 1∶2.5,water content of NADES-11 was 18%,liquid-solid ratio was 30 mL/g,extraction time was 30 min and extraction temperature was 30℃.The extraction efficiency of NADES-11 was significantly higher than that of 50%methanol(P<0.05).IC50 values of NADES-11 extract(261.17 and 744.34μg/mL)to 1,1-diphenyl-2-trinitrophenylhydrazine radical and hydroxyl radical were all lower than those of 50%methanol extract(420.97 and 1175.12μg/mL).Ascorbic acid equivalent antioxidant capacity of NADES-11 extract(17.19 and 360.80 mg VC/g)was higher than that of 50%methanol extract(10.67 and 228.54 mg VC/g).CONCLUSIONS The optimized extraction process of phenylethanol glycosides from C.deserticola using NADESs is environmental,stable and feasible.