双甲脒胶体金免疫快速检测试纸条研制及其在蜂蜜中的应用

Development of amitraz colloidal gold rapid immunoassay strip and its application in honey

目的基于胶体金免疫层析原理研制双甲脒快速检测试纸条,评价其在蜂蜜检测中的应用效果。方法使用双甲脒-牛血清白蛋白偶联物作为人工抗原免疫小鼠获得单克隆抗体,制备胶体金微孔试剂,使用双甲脒-卵清蛋白(ovalbumin,OVA)偶联物和羊抗鼠IgG抗体分别作为试纸条的检测线和控制线,并对蜂蜜样品进行添加检测。结果研制的试纸条可以定性检测蜂蜜中双甲脒的残留,对蜂蜜中双甲脒的检出限为0.2mg/kg,检测用时约25min,检测与双甲脒功能和结构相似的其他药物时无交叉反应,特异性较好。结论本研究研制的试纸条适用于蜂场现场快速检测以及基层实验室中大量样本的筛查,为蜂蜜中抗生素残留控制提供有效监管手段。

Objective To develop a rapid test strip for amitraz by colloidal gold immunochromatography and evaluate its application effect in honey detection.Method A mouse was immunized by using the amitraz-bovine serum albumin conjugate as an artificial antigen to obtain a monoclonal antibody,a colloidal gold microporous reagent was prepared,the amitraz-ovalbumin(OVA)conjugate and the goat anti-mouse IgG antibody were respectively used as a detection line and a control line of a test strip,and addition detection is performed on a honey sample.Result The developed test strip could be used for qualitative detection of amitraz residue in honey,the limit of detection for amitraz in honey was 0.2 mg/kg,and the detection time was about 25 min,there was no cross-reaction for the detection of other drugs similar to amitraz in function and structure,with good specificity.Conclusion The test strip developed in this study is suitable for rapid detection in bee farms and screening of a large number of samples in grass-roots laboratories,and provides effective supervision means for controlling antibiotic residues in honey.