杜仲TCP转录因子鉴定及生物信息学分析

Identification and bioinformatics analysis of TCP transcription factors in Eucommia ulmoides

目的 对杜仲TCP(Teosinte branched 1/Cycloidea/Proliferating)基因家族进行筛选分析,以期为Eu TCPs基因功能深入研究奠定基础。方法 以杜仲基因组数据库为基础,通过生物信息学系统分析TCP基因家族的理化性质、系统进化、基因结构、启动子顺式作用元件及其在杜仲叶片发育及胶形成中的表达水平。结果 从杜仲基因组中共鉴定到14个Eu TCPs,氨基酸数目介于139~492,理论等电点分布在5.54~9.72,相对分子质量区域为18 880~53 620,亚细胞定位预测在细胞核中,均为亲水性蛋白。系统进化分为PCF、CIN和CYC/TB1 3个亚家族,分别包含5、6和3个Eu TCPs蛋白。表达模式分析显示,Eu TCPs在杜仲叶片不同发育时期存在显著差异,每个发育时期Eu TCPs表达量各不相同,Eu TCPs正调控杜仲胶的形成。结论 杜仲中TCP家族包含14个成员,各成员的分子特征和组织表达特异性存在一定的差异,Eu TCPs可能参与杜仲叶片发育及杜仲胶的形成。

Objective In order to provide a basis for EuTCPs gene function research, Eucommia ulmoides TCP(Teosinte branched 1/Cycloidea/Proliferating) gene family was screened and analyzed in this study. Methods Based on E. ulmoides genome database,bioinformatics methods was used to systematically analyze the physical and chemical properties, phylogenetic evolution, gene structure, promoter cis-acting element and their expression in leaf development and gum formation of LBD gene family. Results In this study, a total of 14 EuTCPs were identified from E. ulmoides genome, EuTCPs encodes 139—492 amino acids, and theoretical isoelectric points distribution were 5.54—9.72, molecular weight between 18 880 to 53 620 by physicochemical analysis, all of EuTCPs proteins were hydrophilic proteins and expressed in nucleus. EuTCP gene family was divided into three subfamilies: PCF,CIN and CYC/TB1, which contain five, six and three EuTCPs proteins respectively. Expression pattern analysis showed that EuTCPs played different roles in leaf development of E. ulmoides and positively regulated the formation of gutta-percha. Conclusion In this study, the members of the TCP family were comprehensively identified and bioinformatics analysis from the genomic level of E.ulmoides, which provides basic data for further functional research of EuTCP genes.