桉树无性系BL1号的组织培养技术研究

Study on Tissue Culture Technology of Eucalyptus Clone BL1

以广西国有博白林场林科所选育的桉树无性系BL1号优株带芽茎段为外植体进行组培快繁技术体系研究。阐述了桉树无性系BL1号快速繁殖的过程,通过外植体的采集、消毒、诱导、扩繁、生根、移栽等阶段,培养出完整的植株。结果表明:晴天+纯净水冲洗30 min+0.15%HgCl_(2)(3+7)min为外植体消毒最佳处理方案。在改良的MS培养基添加V;15.0 mg·L^(-1)能有效控制外植体褐变,不同褐化剂对外植体的影响效果为:V;>Cys>Na_(2)S_(2)O_(3)>CK。改良MS+BA 1.0 mg·L^(-1)+KT 0.2 mg·L^(-1)+NAA 0.05 mg·L^(-1)是继代培养的最适培养基。改良MS+BA0.5mg·L^(-1)+NAA 0.08 mg·L^(-1)+KT 0.02 mg·L^(-1)是生根有效芽繁殖最适培养基。改良MS+BA 0.25 mg·L^(-1)+NAA 0.1mg·L^(-1)+IBA0.6 mg·L^(-1)是生根最适培养基。

The tissue culture and rapid propagation technology system of Eucalyptus clone BL1 was studied using stem segments with buds as explants.The process of rapid propagation of BL1 is described in this paper.Cultivation of complete seedling plants of this clone involved many steps including shoot collection,disinfection,induction,propagation,rooting and transplanting of explants.The results showed that the best method of explants collection and treatment involved collection on sunny days,then washing for+30 minutes with pure water+0.15%HgCl_(2)for 3+7 minutes.It was found that V;15.0 mg·L^(-1)was an effective method for controlling explant browning,and the effect of different browning agents on explants is as follows:V;>Cys>Na_(2)S_(2)O_(3)>CK.Modified MS+BA 1.0 mg·L^(-1)+KT 0.2 mg·L^(-1)+NAA 0.05 mg·L^(-1)was the optimal medium for subculture.Modified MS+BA0.5 mg·L^(-1)+NAA 0.08 mg·L^(-1)+KT 0.02 mg·L^(-1)proved an effective medium for bud propagation.The optimal rooting medium was modified MS+BA 0.25 mg·L^(-1)+NAA 0.1mg·L^(-1)+IBA0.6 mg·L^(-1).