低氧胁迫下斑马鱼鳃中核糖体蛋白基因家族的表达分析

Differential expression analysis of the ribosomal protein gene family in zebrafish gills under hypoxia stress

为研究斑马鱼核糖体蛋白应对低氧胁迫的生物学功能,对低氧胁迫和常氧条件下斑马鱼(Danio rerio)鳃组织进行转录组分析,利用高通量测序检测在低氧胁迫与常氧条件下斑马鱼鳃转录组中核糖体蛋白家族基因的表达差异。结果表明:在两个不同浓度的低氧胁迫下,斑马鱼鳃组织中60个核糖体蛋白基因的表达量显著上调,其中,大亚基核糖体蛋白基因35个,小亚基核糖体蛋白基因25个。在低氧胁迫下斑马鱼鳃中显著差异表达基因GO富集的前15条通路中均包括核糖体蛋白基因,且其中的5条通路与核糖体蛋白组装合成相关。在富集到“translation”GO通路中,富集到44个核糖体蛋白基因。另外,利用前期筛选出的低氧条件下斑马鱼鳃中显著低表达的2个miRNAs,针对低氧下表达量显著上调的60个核糖体蛋白基因进行靶基因预测,结果表明:斑马鱼miR-455-3p可以同时靶向核糖体蛋白基因rpl13和rplp1来调控斑马鱼对低氧环境的适应。

To study the biological function of zebrafish ribosomal proteins in response to hypoxic stress, transcriptome analysis was performed on the gill tissues of Danio rerio under hypoxic and normoxic conditions, and the gene expression differences of ribosomal protein family genes in the gill of D. rerio were compared. The results showed that the expression levels of 60 ribosomal protein genes in zebrafish gill tissues were significantly up-regulated at two different concentrations of hypoxia stress, which included 35 large subunit ribosomal protein genes and 25 small subunit ribosomal protein genes. Among the top 15 pathways enriched by the significantly deferentially expressed genes in the zebrafish gills under hypoxia stress, all included the ribosomal protein genes, and 5 were related to ribosomal protein assembly and synthesis. In the enriched “translation” GO term, 44 ribosomal protein genes were included. In addition, the target genes of 60 significantly up-regulated ribosomal protein genes were predicted by two significantly down-expressed miRNA in zebrafish gills under hypoxia stress, which were screened in our previous study. The results show that miR-455-3 p can target both ribosomal protein genes rpl13 and rplp1, which might be involved in the adaptation to the hypoxia environment.