一种改善睡眠配方饮品的功效研究

Study on the Efficacy of a Formula Drink for Improving Sleep

目的:研究一种配方食品“我的小夜曲”对于改善睡眠的功效。方法:随机选取5 dpf野生型AB品系斑马鱼于6孔板中,每孔处理30尾斑马鱼。采用戊四唑(Pentylenetetrazole,PTZ)建立失眠模型,分别水溶给予“我的小夜曲”6.25μL·mL^(-1)、12.50μL·mL^(-1)、25.00μL·mL^(-1)、50.00μL·mL^(-1)和100.00μL·mL^(-1),阳性对照褪黑素125μg·mL^(-1),同时设置正常对照和模型对照,每孔容量为3 mL。除正常对照组外,其余各实验组均水溶给予PTZ建立斑马鱼失眠模型。处理结束后观察统计各实验组斑马鱼的死亡数量和毒性反应情况,确定MTC;取MTC及3个浓度组、阳性对照、模型对照和正常对照,将斑马鱼转移至96孔板中,200μL/尾,1尾/孔,利用行为分析仪检测斑马鱼的觉醒活动时间和觉醒活动量。结果:各组中均无死亡,但与对照组相比,高浓度两组状态较差,故将MTC定为25.0μL·mL^(-1)。观察时间内,空白对照与模型组比较,觉醒活动时间有显著差异(p<0.001)。与模型组比较,褪黑素组及各处理组均显著下调斑马鱼的觉醒活动时间(p<0.01),处理组睡眠改善作用依次为59%、43%和43%。结论:“我的小夜曲”对失眠症状具有明显的改善作用。

Objective:To study the effect of a formula drink“My Serenade”on improving sleep.Method:5 dpf wild type AB zebrafish were randomly selected into 6-well plates,and 30 zebrafish were treated in each well.The insomnia model was established by pentylenetetrazole(PTZ).The concentration of“My Serenade”was 6.25μL·mL^(-1),12.50μL·mL^(-1),25.00μL·mL^(-1),50.00μL·mL^(-1) and 100.00μL·mL^(-1) which was dissolved in water,and the concentration of positive control melatonin was 125μg·mL^(-1).The normal control and model control were set and the volume of each well was 3 mL.Experimental groups were given PTZ to establish insomnia model of zebrafish.After treatment,counted the death number and observed toxic reaction of zebrafish in each experimental group,and determined the MTC;chose MTC and three concentration groups,positive control,model control and normal control,zebrafish were transferred to a 96-well plate,200μL/tail,1 tail/well.Awakening time and awakening move distance of zebrafish were detected by behavior analyzer.Result:There was no death in all groups,but the two high concentration groups’status were worse than those in the control group,so the MTC was set as 25.0μL·mL^(-1).During the observation period,there was a significant difference in awakening activity time between the blank control group and the model group(p<0.001).Compared with model group,melatonin control and treatment groups significantly down-regulated the arousal time(p<0.01),the sleep improvement effect of treatment group was 59%,43%and 43%,respectively.Conclusion:“My Serenade”can significantly improve insomnia symptoms.