Piwil2蛋白在乳腺恶性叶状肿瘤组织中的表达及临床预后分析

Study on the expression of Piwil2 protein in malignant phyllodes tumor tissue and its clinical prognosis

目的:探讨Piwil2蛋白在乳腺恶性叶状肿瘤组织中的表达,分析Piwil2蛋白的表达与乳腺恶性叶状肿瘤相关临床病理因素之间的关系及对病人10年生存预后的影响。方法:收集乳腺恶性叶状肿瘤女性病人资料50例,同时选取因乳腺炎切除的乳腺正常组织70例作为对照组。采用免疫组织化学法分别检测Piwil2蛋白在乳腺恶性叶状肿瘤、正常乳腺组织中的表达。结果:Piwil2蛋白在乳腺恶性叶状肿瘤组织中的阳性表达率为86.00%,明显高于正常乳腺组织的0.00%,差异有统计学意义(χ^(2)=93.82,P<0.01)。Piwil2蛋白的阳性表达率与病人肿瘤直径≥10 cm、Ki-67≥25%、瘤细胞异型性、CyclinD1蛋白阳性表达及核分裂≥15个/10HPF有关(P<0.05~P<0.01),而与病人年龄及术后是否复发无关(P>0.05)。乳腺恶性叶状肿瘤Piwil2蛋白阳性表达的病人10年生存率(65.12%)低于Piwil2蛋白阴性表达病人(100.00%),差异有统计学意义(χ^(2)=3.95,P<0.05)。结论:Piwil2蛋白的异常表达参与了乳腺恶性叶状肿瘤的演变、发展,也可作为临床预测病人生存预后的参考指标。

Objective:To investigate the expression levels of Piwil2 protein in breast malignant lobar tumor tissues,and analyze the relationship between Piwil2 protein expression and clinicopathological factors related to breast malignant lobar tumor and its influence on 10-year survival and prognosis of patients.Methods:The clinical data of 50 female patients with malignant lobular tumor of breast were collected,and 70 cases of normal breast tissue resected due to mastitis were selected as the control group.The expression levels of Piwil2 protein in malignant lobar tumor and normal breast tissues were detected by EnVisionTM immunohistochemistry.Results:The positive expression rate of Piwil2 protein in malignant lobar tumor tissue was 86.00%,which was significantly higher than that in normal breast tissue(0.00%)(χ^(2)=93.82,P<0.01).The positive expression of Piwil2 protein in malignant lobar tumor tissue was related to the tumor diameter≥10 cm,Ki-67≥25%,oncocyte atypia,CyclinD1 protein positive expression and Karyokinesis≥15/10HPF(P<0.05 to P<0.01),but not related to the age and postoperative recurrence(P>0.05).The ten-year survival rate of malignant lobar tumor patients with positive expression of Piwil2 protein(65.12%)was lower than that of patients with negative expression of Piwil2 protein(100.00%),and the difference of which was statistically significant(χ^(2)=3.95,P<0.05).Conclusions:The abnormal expression of Piwil2 protein is involved in the evolution and development of malignant breast lobar tumor,and can also be used as a reference index for predicting the survival and prognosis of patients.