HPVE7蛋白、sHLA-G、STNFR在宫颈癌及癌前病变中检测价值

Detection value of HPVE7 protein,sHLA-G and STNFR in cervical cancer and precancerous lesions

目的 探讨人乳头瘤病毒(HPV)E7蛋白、可溶性白细胞G抗原(sHLA-G)、可溶性肿瘤坏死因子受体(STNFR)Ⅰ、可溶性肿瘤坏死因子受体(STNFR)Ⅱ在宫颈癌及癌前病变中的检测价值。方法 选取2018年11月至2020年12月驻马店市中医院宫颈癌患者119例及宫颈癌前病变患者119例。取清晨空腹静脉血,酶联免疫吸附试验检测血清sHLA-G、STNFR、HPVE7,比较两组HPVE7蛋白、sHLA-G、STNFR的表达水平及不同分期、不同分化程度患者血清sHLA-G、STNFR、HPVE7检测值,分析HPVE7蛋白、sHLA-G、STNFR水平与临床分期及分化程度相关性,并采用受试者工作特征曲线(ROC)分析血清对宫颈癌诊断价值。结果 宫颈癌组HPVE7蛋白、sHLA-G、STNFRⅠ水平高于癌前病变组,差异有统计学意义(P<0.05)。HPVE7蛋白、sHLA-G、STNFRⅠ水平与疾病分期呈正相关,HPVE7蛋白、sHLA-G、STNFRⅠ水平与疾病分化程度呈负相关(P<0.05)。HPVE7蛋白、sHLA-G、STNFRⅠ联合诊断宫颈癌的AUC为:0.880、敏感性为88.24%、特异性为79.83%(P<0.05)。结论 HPVE7蛋白、sHLA-G、STNFRⅠ在宫颈癌患者中异常表达,与其病情密切相关,联合检测可作为疾病诊断一种辅助性方法。

Objective To investigate the effects of human papillomavirus(HPV)E7 protein,soluble leukocyte G antigen(sHLA-G),soluble tumor necrosis factor receptor(STNFR)I and soluble tumor necrosis factor receptor(STNFR)Ⅱ in cervical cancer and detection value in precancerous lesions. Methods A total of 119 patients with cervical cancer and 119 patients with cervical precancerous lesions in Zhumadian Hospital of Traditional Chinese Medicine from November 2018 to December 2020 were selected. Fasting venous blood was collected, and serum sHLA-G, STNFR, and HPVE7 were detected by enzyme-linked immunosorbent assay. The expression levels of HPVE7 protein,sHLA-G,and STNFR between the two groups were compared,and the serum sHLA-G and STNFR levels of patients with different stages and degrees of differentiation were compared. The correlation between the levels of HPVE7 protein,sHLA-G,STNFR and clinical stage and differentiation degree was analyzed,and the receiver operating characteristic curve(ROC)was used to analyze the diagnostic value of serum for cervical cancer. Results The levels of HPVE7 protein,sHLA-G and STNFRⅠ in the cervical cancer group were higher than those in the precancerous lesion group,the difference was statistically significant(P<0.05). There were statistically significant differences in the levels of HPVE7 protein,s HLA-G,and STNFRⅠ among cervical cancer patients with different stages and degrees of differentiation(P<0.05),while there was no statistical difference in the levels of STNFR Ⅱ and between different stages,different degrees of differentiation,and different groups(P>0.05). The levels of HPVE7protein,s HLA-G and STNFRⅠwere positively correlated with disease stage,and the levels of HPVE7 protein,sHLA-G and STNFRI were negatively correlated with the degree of disease differentiation. The AUC of the combination of HPVE7 protein,sHLA-G and STNFRI in the diagnosis of cervical cancer was:0.880,the sensitivity was 88.24%,and the specificity was 79.83%(P<0.05). Conclusion HPVE7 protein,sHLA-G and STNFRⅠ are abnormally expressed in patients with cervical cancer,which are closely related to the disease.Combined detection can be used as an auxiliary method for disease diagnosis.