969例呼吸道感染儿童中人博卡病毒的感染情况与临床特征分析

Analysis of the infection and clinical characteristics of human Bocavirus in 969 children with respiratory tract infection

目的了解遵义地区儿童急性呼吸道感染中人博卡病毒(HBoV)的感染情况,为儿童呼吸道感染的临床诊疗提供参考。方法收集2019年5月至7月遵义市第一人民医院急性呼吸道感染儿童鼻咽拭子标本969例,采用PCR-荧光探针法检测HBoV核酸,对PCR产物进行Sanger法测序,并收集感染患儿临床资料进行临床特征分析。结果在969例鼻咽拭子标本中,共检出45例阳性标本,阳性率为4.6%。其中男性患儿多于女性,两者检出率比较,差异无统计学意义(P>0.05);阳性病例主要集中在36月以内患儿(77.7%),不同年龄段检出率比较,差异有统计学意义(P<0.05);不同年龄段阳性患儿的临床特征有所差异,其中各年龄段出现发热、扁桃体肿大、CRP增高的比例比较,差异有统计学意义(P<0.05);阳性患儿的临床诊断以社区获得性肺炎最多见(51.1%);单纯HBoV感染率为37.8%,混合感染比例高达62.2%。结论HBoV是2019年5至7月遵义地区儿童急性呼吸道感染的重要病原体,及时准确的检测和分析HBoV感染,对临床诊疗具有重要意义。

Objective To investigate tthe infection situation of human Bocavirus(HBoV)in children with acute respiratory tract infection in Zunyi area,and to provide reference for clinical diagnosis and treatment of children with related respiratory tract infection.Methods A total of 969 nasopharyngeal swab samples from children with acute respiratory tract infection were collected from the First People’s Hospital of Zunyi from May to July 2019.HBoV nucleic acid was detected by PCR-fluorescent probe method,PCR products were sequenced by Sanger method,and clinical data of infected children were collected for clinical characteristics analysis.Results Among the 969 nasopharyngeal swab specimens,a total of 45 positive specimens were detected,with a positive rate of 4.6%.Among them,male patients were more than female patients,with no statistical significance(P>0.05).The positive cases were mainly in children less than 36 months(77.7%).There was a statistically significant difference in the detection rates of different age groups(P<0.05).The clinical characteristics of positive children in different age groups were different,among which,the proportion of fever,tonsillar enlargement and increased CRP in different age groups was statistically significant(P<0.05).Community acquired pneumonia was the most common clinical diagnosis in positive children(51.1%).The infection rate of HBo V alone was 37.8%,and the rate of mixed infection was up to 62.2%.Conclusion HBo V is an important pathogen of acute respiratory tract infection in children in Zunyi area from May to July 2019.Timely and accurate detection and analysis of HBo V infection is of great significance for clinical diagnosis and treatment.