腊花组培快繁体系研究

Study on Tissue Culture and Rapid Propagation System of Chamelaucium uncinatum

为探讨最适初代诱导、增殖继代生根的培养基配方,建立腊花的组织培养技术体系,以腊花嫩茎尖为实验材料,采用MS培养基,向培养基中添加不同配比组合的KT和NAA,对腊花进行初代诱导培养;设置6-BA和NAA不同激素浓度组合进行增殖培养;选用1/2MS培养基为腊花生根培养的基础培养基,添加IBA不同浓度配比培养实验。最佳初代诱芽培养基为MS+KT 3.0 mg/L+NAA 0.2 mg/L+琼脂6.2 g/L+蔗糖30 g/L,pH 5.8,诱导率达88.8%。最佳增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+琼脂6.2 g/L+蔗糖30 g/L,pH 5.8,增殖系数为3.6。最佳生根培养基为1/2MS+IBA 0.6 mg/L+琼脂6.2 g/L+蔗糖30 g/L+0.1 g/L活性炭,pH 5.8,生根率100%。

To find out the best medium for inducing the first generation and multiplying the second generation and rooting, the tissue culture technique system of Chamelaucium uncinatum was established. The tender shoot tip of C. uncinatum was used as the experimental material, MS medium was used to add KT and NAA with different proportions, the first generation of C. uncinatum was induced, different concentrations of 6-BA and NAA were combined for proliferation, the 1/2MS medium was selected as the basic medium for the rooting culture of C. uncinatum, and different concentrations of IBA were added to the medium. The best medium for bud induction was MS+ KT 3.0 mg/L+ NAA 0.2 mg/L+ agar 6.2 g/L+ sucrose 30 g/L with pH 5.8, and the induction rate was 88.8%. The best medium for proliferation was MS+ 6-BA 1.0 mg/L+ NAA 0.1 mg/L+agar 6.2 g/L+ sucrose 30 g/L with pH 5.8, and the proliferation coefficient was 3.6. The best rooting medium was 1/2MS+ IBA 0.6 mg/L+ agar 6.2 g/L+ sucrose 30 g/L+ 0.1 g/L activated carbon with pH 5.8, and the rooting rate was 100%.