泰山白首乌根际土壤真菌多样性分析

Sequencing Analysis of Fungal Diversity in Rhizosphere Soil of Cynanchum bungei Decne

为了解泰山白首乌根际土壤微生物群落结构和组成,充分利用根际微生物资源,采用Illumina MiSeq高通量测序技术,分析测定来自山东省莱芜市莲花山(LW)和济南市历下区(LX)、长清区(CQ)3个产地的泰山白首乌根茎10和20 cm深度根际土壤真菌群落的丰富度和多样性。结果表明,6个样品共得到真菌1589个OTUs,分属于11门35纲84目142科237属。子囊菌门(Ascomycota)和被孢霉门(Mortierellomycota)是6个样品共同的优势菌门。样品CQ20的群落丰度和多样性最高,样品LX20的真菌群落分布最均匀;随着地下根茎深度加深,真菌微生物群落的丰度和多样性增大。不同产地和根茎深度样品间土壤真菌群落存在显著差异,上述结果为优选根际促生菌、提高仿野生种植技术提供了理论依据。

To understand the structure and composition of rhizosphere soil microbial community of Cynanchum bungei Decne,and make full use of rhizosphere microbial resources,the fungal community richness and diversity in rhizosphere soil(at 10 and 20 cm deep)of Cynanchum bungei Decne form Lianhuashan of Laiwu City(LW),Lixia(LX)and Changqing(CQ)of Jinan City of Shandong Province were analyzed by Illumina MiSeq high throughput sequencing technology.The results showed that 1589 OTUs were obtained from 6samples,belonging to 11 phyla,35 classes,84 orders,142 families and 237 genera.Ascomycota and Mortierellomycota were the common dominant bacteria of 6 samples at phyla level.Sample CQ20 had the highest community abundance and diversity,and sample LX20 had the most uniform distribution of fungal community.With the deepening of underground rhizome depth,the abundance and diversity of fungal microbial community increased,which provided theoretical basis for optimizing the rhizosphere growth-promoting bacteria and improving the planting technique of simulating wild.