IL-33、IL1RL1基因多态性与北方高寒地区汉族人群原发性高血压合并冠心病的相关性研究

Association between IL-33, IL1RL1 gene polymorphism and essential hypertension complicated with coronary heart diseasein Han population in northern alpine area

目的探讨IL-33、IL1RL1基因单核苷酸多态性与北方高寒地区汉族人群原发性高血压合并冠心病发病风险之间的关联性。方法选择2018年1月~2020年12月在齐齐哈尔市中医医院资料完整、成功完成基因分型者。根据原发性高血压及冠心病的诊断标准,选择该院心内科住院确诊患者(病例组)和体检中心的健康体检者(对照组)各80例,采用多重连接检测反应方法对IL33基因位点rs7025417、IL1RL1基因位点rs1041973基因分型进行检测,分析组间基因分布频率差异,应用二元多因素Logistic回归分析各位点与疾病的关联性。结果通过rs7025417位点基因型分布频率发现,疾病组rs7025417的TT基因型和T型等位基因分布频率显著高于对照组(P<0.01);二元多因素Logistic回归分析发现,rs7025417位点的TT基因型是原发性高血压合并冠心病发病的独立危险因素[OR=2.421,95%CI(1.028,5.699),P=0.043]。而rs1041973基因分型无组间差异。结论rs7025417多态性与中国北方高寒地区汉族人群原发性高血压合并冠心病相关,rs7025417位点的T等位基因携带者,尤其是TT基因型携带者发病风险更高。

Objective To investigate the association between single nucleotide polymorphisms(SNPS) of IL-33 and IL1RL1 genes and the risk of essential hypertension(EH) complicated with coronary heart disease(CHD)in Han population in northern alpine area. Methods Patients with complete data and successful genotyping were selected from Jan 2018 to Dec 2020 in Qiqihar Hospital of Traditional Chinese Medicine. According to the diagnosis standard of EH and CHD, 80 inpatients diagnosed in the department of cardiology of the hospital(case group) and 80 healthy subjects in the physical examination center(control group) were selected. The polymorphic locus rs7025417of IL-33 gene and rs1041973 of IL1RL1 gene were genotyping by multiple linkage detection response method. The differences of gene distribution frequency between groups were analyzed, and the association between each gene locus and disease was analyzed by binary logistic regression. Results In the distribution frequency of rs7025417genotype of IL-33 gene, it was found that the distribution frequency of TT genotype and T allele of rs7025417 in case group was significantly higher than that in control group(P<0.01). The TT genotype of rs7025417 was found to be an independent risk factor for essential hypertension complicated with coronary heart disease by binary logistic regression analysis [OR=2.421, 95%CI(1.028, 5.699), P=0.043]. There was no difference in genotype of IL1RL1 gene rs1041973. Conclusion rs7025417 polymorphism is associated with the risk of essential hypertension complicated with coronary heart disease in Han population in northern alpine region. T allele carriers of rs7025417 locus, especially TT genotype carriers, have a higher risk of disease.