高效液相色谱检测冷冻饮品中赤藓红方法探讨

Determination of erythritol in frozen drink samples by high performance liquid chromatography

目的 建立1种冷冻饮品中赤藓红简单、准确的高效液相色谱检测法。方法 采用聚酰胺吸附样品中的赤藓红,乙醇-氨水-水溶液(7+2+1)解吸,解吸液蒸发至近干,甲醇-甲酸(6+4)复溶,微孔滤膜过滤后进行高效液相色谱仪检测。C18反相键合固定相色谱柱(250 mm×4.6 mm, 5μm)分离,20 mmol/L乙酸铵水溶液和甲醇为流动相,二极管阵列检测器530 nm作为检测波长,外标法定量。结果 赤藓红在质量浓度0.2~10.0 mg/L范围内线性良好(r>0.999);在冷冻饮品中加入低、中、高3个质量浓度水平标准溶液,加标回收率为89.9%~95.5%,相对标准偏差(RSD)均<5.0%。方法检出限为0.1 mg/kg,定量限为0.3 mg/kg,满足国标要求。结论 建立的方法能够用于冷冻饮品中赤藓红测定,方便快捷,准确可靠。

Objective To establish a simple and accurate high performance liquid chromatography(HPLC) method for the determination of erythrosine in frozen drink samples. Methods The erythrosine in samples was absorbed by polyamide, desorbed by ethanol-ammonia-water(7+2+1) mixed solution, evaporated until nearly dry, and re-dissolved by methanol-formic acid(6+4),followed by filtration by microporous membrane, and sent to HPLC for analysis.The separation was performed on a C18 reverse-phase bonded stationary column(250 mm×4.6 mm, 5 μm) with 20 mmol/L ammonium acetate aqueous solution and methanol as mobile phase, and detected by a diode array detector at wavelength of 530 nm.The external standard method was used for quantitative analysis. Results The linearity of erythrosine was good in the mass concentration range of 0.2-10.0 mg/L(r>0.999).The average spiked recoveries of low medium and high levels were 89.9%-95.5% with relative standard deviations(RSD) <5.0%.The limit of detection was 0.1 mg/kg, and the limit of quantification was 0.3 mg/kg, which met the requirements of national standard. Conclusion The established method can be used for the determination of erythrosine in frozen drinks, and the results are accurate and reliable.