不同光照条件对斑马鱼趋光行为的影响

Effects of different light conditions on phototactic behavior of zebrafish

为探究斑马鱼(Danio rerio)在不同光照条件下的趋光行为差异,记录了6月龄的斑马鱼成鱼在紫外光(ultraviolet, UV)和可见光照射下的行为反应和在无光、紫光(420nm)、蓝光(460nm)、绿光(500nm)、黄光(585nm)和红光(620nm)6种光照条件下的趋光分布情况。结果表明,刺激光为UV时,斑马鱼优先游向黑暗环境一侧,刺激光为可见光时,斑马鱼优先游向可见光一侧;UV和可见光分别设置在试验区A和试验区B时,随着UV刺激强度增加,斑马鱼的偏好指数未呈现明显的增加趋势,UV和可见光同时设置在试验区B时,随着UV刺激强度增加,斑马鱼的偏好指数呈现出明显的下降趋势;5min和30min时斑马鱼的平均分布率均呈现红光区>紫光区>蓝光区>黄光区,绿光区的平均分布率在5min时高于红光区,在30min时介于紫光区和蓝光区之间。研究表明,斑马鱼趋向可见光而远离紫外光,表现出明显的避UV性和趋可见光性,且避UV性与趋可见光性之间存在显著的抑制作用,而无协同作用。此外,斑马鱼对红光、绿光和紫光的喜好程度较高,对黄光的喜好程度较低。研究结果可为斑马鱼视觉生态的研究提供参考。

In order to explore the difference of phototactic behaviors of zebrafish under different light conditions, the behavior responses of 6-month-old adult zebrafish under ultraviolet light and visible light were recorded, and the distribution of zebrafish under five monochromatic lights(purple, blue, green, yellow and red) and dark condition were also recorded. The results showed that when the stimulus light was UV, zebrafish preferred to swim to the dark side, and when the stimulus light was visible light, zebrafish preferred to swim to the visible light side. When UV and visible light were set in test area A and test area B respectively, the preference index of zebrafish did not increase with the increase of UV stimulation intensity, while when UV and visible light were set in test area B at the same time, the preference index of zebrafish showed a significant downward trend with the increase of UV stimulation intensity. The average distribution rate of zebrafish in red light in 5 min and 30 min was higher than that in purple light, in blue light, and in yellow light by sequence. The average distribution rate of green light in 5 min was higher than that in red light, and the average distribution rate of green light in 30 min was higher than that in blue light and lower than that in purple light. The results revealed that zebrafish tended to visible light and stayed away from ultraviolet light, showing obvious UV avoidance and phototaxis, and there was a significant inhibiting effect between UV avoidance and phototaxis, but there was no synergist between them. In addition, zebrafish had a higher preference for red light, green light and purple light, but lower preference for yellow light. The results can provide reference for the research of visual ecology of zebrafish.