高脂饮食诱导沙鼠非酒精性脂肪肝性纤维化模型的建立

Establishment of non-alcoholic fatty liver disease-derived fibrosis induced by high-fat diet in gerbils

目的 构建高脂膳食诱导的沙鼠非酒精性脂肪肝性纤维化模型。方法 30只40~60 g雄性沙鼠随机采用高脂饮食(模型组)或普通饮食(对照组)喂养16周,测定血清和肝组织中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-c)、高密度脂蛋白胆固醇(HDL-c)水平,HE、油红O、Masson染色观察肝脂肪变性和纤维化程度。结果模型组体质量及TC、TG、LDL-c水平均高于对照组(P<0.05);病理检查显示肝细胞充满脂滴,汇管区出现纤维化。结论高脂饮食可构建稳定的非酒精性脂肪肝性纤维化沙鼠模型。

Objective To establish a gerbil model of non-alcoholic fatty liver disease(NAFLD)-derived fibrosis induced by high-fat diet(HFD). Methods Thirty male gerbils weighed 40-60 g were randomly fed with HFD(model group) or normal diet(control group) for 16 weeks. Serum and hepatic levels of triglyceride(TG), total cholesterol(TC), low density lipoprotein cholesterol(LDL-c), and high density lipoprotein cholesterol(HDL-c) were measured. Hepatic steatosis and fibrosis were observed by HE, oil red O, and Masson stain. Results Body weight and TC, TG, LDL-c levels were higher in model group than in control group(P<0.05). Pathological examination showed numerous lipid droplets in hepatocytes and fibrosis in the portal area. Conclusion HFD can induce a stable gerbil model of NAFLD-derived fibrosis.