^(60)Co-γ射线辐照对草地贪夜蛾自交种群发育与繁殖的影响

Effects of ^(60)Co-γirradiation on the development and reproduction of inbreeding population of Spodoptera frugiperda

为了解^(60)Co-γ射线辐照对草地贪夜蛾Spodoptera frugiperda成虫发育与繁殖的影响,本文以草地贪夜蛾6日龄蛹为对象,比较不同辐照剂量下草地贪夜蛾自交种群的羽化率、翅畸形率、寿命、产卵量以及子代卵孵化率的差异。结果表明,草地贪夜蛾成虫羽化率随着辐照剂量的升高而呈现降低的趋势,其中250 Gy、350 Gy、400 Gy以及500 Gy剂量辐照下的羽化率分别降低至72.8%、69.4%、69.4%和50.6%,与对照组(91.7%)相比差异显著;翅畸形率在400 Gy时值最高,为25.6%,但与对照组相比并无显著差异;其雌、雄成虫平均寿命均随辐照剂量的升高而缩短,且辐照后处理组雌、雄成虫寿命均显著低于对照组成虫寿命;雄虫日存活率在100 Gy、200 Gy、300 Gy和500 Gy处理条件下高于雌虫日存活率,而350 Gy和400 Gy处理组,以及对照组雌虫日存活率高于雄虫。草地贪夜蛾种群产卵雌虫数、单雌产卵量、及子代孵化情况与对照组相比差异显著,300~500 Gy的辐照剂量下,亲代没有卵粒产出,而100 Gy至250 Gy的辐照剂量下,产卵量显著降低,且在100 Gy时处理组的卵中有少量孵化。本文对草地贪夜蛾辐照不育剂量进行了初步评价,为今后实现该虫不育技术防治提供科学参考。

In order to understand the effects of ^(60)Co-γradiation on the development and reproduction of the adult Spodoptera frugiperda,6 day old pupae were irradiated with different doses of ^(60)Co-γradiation to compare the differences on the adult emergence,wing deformity,longevity,egg production and offspring hatching rate of S.frugiperda in inbreeding population.Results showed that,emergence rate of adult S.frugiperda decreased gradually with the increase of irradiation dose,and emergence rate under 250 Gy,350 Gy,400 Gy and 500 Gy irradiation was significantly different from control group(91.7%),which decreased into 72.8%,69.4%,69.4% and 50.6%,respectively.The rate of wing deformity was highest at 400 Gy with a value of 25.6%,was no significantly different founded from control group.Longevity of both female and male adults decreased with increasing irradiation doses,and the longevity of both female and male adults in the control group was significantly higher than treatment groups with different doses(7 doses).Under treatment of 100 Gy,200 Gy,300 Gy and 500 Gy,daily survival rate of male adults was higher than female adults,while daily survival rate of female adults in 350 Gy,400 Gy and control groups was higher than male adults.Compared with the control,there were significant differences in the number of females laying eggs,number of eggs laid by single females and hatching rate of their progeny in S.frugiperda population.By the treatment of 300~500 Gy radiation doses,there were no eggs produced by the parents,while under 100~250 Gy radiation doses,the number of eggs laid by the parents decreased significantly,and a small amount of eggs hatched in the 100 Gy treatment group.In this study,the radiation sterility dose of S.frugiperda was preliminarily investigated,which provided a scientific basis for the realization of sterility control technology of S.frugiperda in future.