基于自然寄主的稻虱缨小蜂规模化饲养的条件优化

Optimization of mass rearing of Anagrus nilaparvatae Pang et Wang on natural hosts

稻虱缨小蜂Anagrus nilaparvatae Pang et Wang是稻飞虱卵期的主要寄生性天敌,是调控稻飞虱种群密度的关键因子。本研究用正交试验设计的方法,对影响稻虱缨小蜂及其自然寄主褐飞虱Nilaparvata lugens产卵的主要因子(温度、光照强度、水稻品种、水稻苗龄、寄主卵龄以及接蜂雌雄比)进行分析和比较,优化带褐飞虱卵苗生产和稻虱缨小蜂生产的方法和条件。生产带卵苗时,25℃强光照条件下用10日龄的黄华占稻苗为佳,扩繁稻虱缨小蜂时,30℃强光照条件下用1日龄的褐飞虱卵接入雌雄比5∶3的蜂为佳,平均产蜂量可达481.3头/盆。以此为基础建立四室繁蜂法进行稻虱缨小蜂规模化饲养,该技术能够进行连续饲养,并提供同一发育阶段产品,稻虱缨小蜂培养与收集室在18 m^(2)的规模下,每批次可以生产约58万头蛹或成蜂,且有提升空间。

The egg parasitoid Anagrus nilaparvatae Pang et Wang,major natural enemy of rice planthopper,was the key factor to control the population density of rice planthopper.The orthogonal experiment was used to study the effects of temperatures,strengths of illumination,rice varieties,rice seedling ages,host egg ages and inoculation female-male proportions on the oviposition of A.nilaparvatae and Nilaparvata lugens.Based on those results,the methods to produce seedlings with eggs of brown planthopper and parasitoids were established and optimized.Accordingly,we concluded that it would be better to use 10-day-old rice plant Huanghuazhan in the condition of strong irradiation intensity at 25℃ to produce seedlings with eggs,and it would be better to import 1-day-old brown planthopper's eggs into 5∶3 female-male proportion of parasitoid in the condition of strong irradiation intensity at 30℃ to propagate the parasitic wasps,which yields 481.3 parasitoid per-tub.Furthermore,a mass rearing method of A.nilaparvatae based on four culture rooms was established,which could achieve continuously rear and provide same development stage A.nilaparvatae.Our experiment results showed that at least 580 thousand A.nilaparvatae pupae or adults were produced in 18 m^(2) rearing and collection room every batch.