[苄基-^(14)C]-毒氟磷在大豆中的残留分布和代谢产物动态变化

Residue Distribution and Dynamics of [benzly-^(14)C]- Dufulin and Metabolites in Soybeans

为深入了解毒氟磷在植物体内的残留代谢规律,以大豆为研究对象,利用;C示踪法研究了[苄基-;C]-毒氟磷在大豆中的残留分布特征及代谢产物动态变化规律。结果表明,施药后毒氟磷主要残留在大豆受施叶上,在豆叶中毒氟磷母体的半衰期为15.32 d,施药后代谢产物的残留浓度均随时间推移而不断增加;至施药20 d时,叶片上毒氟磷母体的残留浓度为6.960 mg·kg^(-1),占总放射性活度的53.443%;代谢产物M4、M3、M1+M2的残留浓度分别为3.838、2.431、1.464 mg·kg^(-1);收获时,可食豆子中残留物含量小于总放射性残留的0.3%,其代谢产物组成与叶中相同;母体及M4的相对含量分别为51.932%和20.301%,且两者残留浓度均大于0.050 mg·kg^(-1)。本试验为客观评价毒氟磷的安全性提供了重要的代谢数据。

To further comprehend the residue and metabolism of Dufulin in plants, the residual distribution characteristics and metabolite dynamics of [benzyl-^(14)C]-Dufulin were applied to soybeans(Glycine max) by the ^(14)C tracing method. The results revealed that, after treatment, Dufulin mainly retained in treated leaves. The half-life of Dufulin parent in soybean leaves was 15.32 d, and the residual concentrations of metabolites increased over time;20 days after treatment, the results proved that the residual concentration of Dufulin in leaves was 6.960 mg·kg^(-1), accounting for 53.443% of the total radioactivity. The residual concentrations of metabolites: M4, M3 and M1+M2 were 3.838, 2.431 and 1.464 mg·kg^(-1), respectively. At harvest, the residual contents in edible beans did not exceed 0.3% of the total radioactive residue, and its metabolites were identical to those in leaves. The relative contents of parent and M4 were 51.932% and 20.301%, and both of the residual concentrations exceeded 0.050 mg·kg^(-1) at harvest. This study provides significant metabolic data for objectively assessing the safety of Dufulin.