摘要
1,3-丙二醇(1,3-propanediol,1,3-PD)可用于工业合成多种化合物,包括聚酯、聚醚和聚氨酯。发酵法生产1,3-PD具有巨大潜力。本文从代谢途径分析入手,梳理了肺炎克雷伯氏菌厌氧代谢途径的相关酶及催化作用,较详细地综述了其产1,3-PD关键酶的分子改造、基因工程菌株的构建和关键酶基因表达、副产物相关代谢酶基因敲除等方面的最新进展,并展望了其今后的发展前景。
1,3-propanediol(1,3-PD)can be used for chemical synthesis of a variety of compounds,including polyesters,polyethers and polyurethanes.Fermentation has great potential to improve 1,3-PD production.This study summarized the enzymes in the anaerobic metabolic pathways of Klebsiella pneumoniae and the catalysis,and reviewed the molecular modification of key enzymes in 1,3-PD production of K.pneumoniae,construction of genetically engineered strains and the expression of key enzyme genes,and knockout of by-product-related metabolic enzyme genes.Moreover,we also described the development prospects of 1,3-PD.
作者
王世伟
王卿惠
向文胜
郝健
李婷婷
赵晨晨
周智博
WANG Shiwei;WANG Qinghui;XIANG Wensheng;HAO Jian;LI Tingting;ZHAO Chenchen;ZHOU Zhibo(Key Laboratory of Resistance Gene Engineering and Preservation of Biodiversity in Cold Areas,College of Life Sciences and Agriculture and Forestry,Qiqihar University,Qiqihar 161005,Heilongjiang,China;School of Life Science,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;Lab of Biorefinery,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2022年第6期2362-2377,共16页
Microbiology China
基金
2021年度黑龙江省大学生创新创业训练计划项目(202110232101)。