提高肺炎克雷伯氏菌产1,3-丙二醇的分子生物学方法研究进展

Molecular biological methods for improving the production of 1,3-propanediol by Klebsiella pneumonia:a review

1,3-丙二醇(1,3-propanediol,1,3-PD)可用于工业合成多种化合物,包括聚酯、聚醚和聚氨酯。发酵法生产1,3-PD具有巨大潜力。本文从代谢途径分析入手,梳理了肺炎克雷伯氏菌厌氧代谢途径的相关酶及催化作用,较详细地综述了其产1,3-PD关键酶的分子改造、基因工程菌株的构建和关键酶基因表达、副产物相关代谢酶基因敲除等方面的最新进展,并展望了其今后的发展前景。

1,3-propanediol(1,3-PD)can be used for chemical synthesis of a variety of compounds,including polyesters,polyethers and polyurethanes.Fermentation has great potential to improve 1,3-PD production.This study summarized the enzymes in the anaerobic metabolic pathways of Klebsiella pneumoniae and the catalysis,and reviewed the molecular modification of key enzymes in 1,3-PD production of K.pneumoniae,construction of genetically engineered strains and the expression of key enzyme genes,and knockout of by-product-related metabolic enzyme genes.Moreover,we also described the development prospects of 1,3-PD.