LncRNA XLOC015132对山羊黑色素沉积的影响及其lncRNA-miRNA-mRNA轴的预测

Effect of LncRNA XLOC_15132 Effect on Pigmentation Accumulation and the Prediction of Its lncRNA-miRNA-mRNA Axis

LncRNA在哺乳动物的基因组中被广泛转录,能够通过相应的miRNA和靶基因促进黑色素瘤的侵袭和转移。为探究LncRNA XLOC_015132的组织表达谱及其与黑色素沉积之间的关系,以酉州乌羊和酉州本地白山羊为试验对象,检测LncRNA XLOC_015132在各组织中以及黑色素瘤细胞中的表达情况;同时,通过靶miRNA和靶基因预测,构建lncRNA-miRNA-mRNA调控网络。qRT-PCR结果显示,LncRNA XLOC_015132在酉州乌羊脾脏、肺、肾脏、大脑、皮肤等组织中的表达量显著高于本地白山羊(P<0.05);LncRNA XLOC_015132在B16-F10黑色素瘤细胞中的定量分析结果显示,随着B16-F10细胞的增殖,LncRNA XLOC_015132表达量呈递增趋势;LncRNA XLOC_015132与互作分子的生物信息学分析结果显示,LncRNA XLOC_015132/miR-150-5p/CCND2轴可能通过激活PI3K-AKT、Wnt等信号通路,在黑色素沉积中发挥重要作用。综合上述结果,LncRNA XLOC_015132的表达与黑色素沉积可能存在正相关关系。本研究结果为深入探究山羊皮肤黑色素沉积的lncRNA调控理论研究提供了基础。

LncRNA was widely transcribed in the mammalian genome and promoted the invasion and metastasis of melanoma through its corresponding miRNA and target genes. To further explore the expression characteristics and melanogenesis function of LncRNA XLOC_015132, the expression profiles of lncRNA XLOC_015132 in different tissues of Youzhou dark goat and Youzhou blank white goat were detected using qRT-PCR. Meanwhile, the expression characteristics of lucRNA XLOC_015132 were detected in B16 cells at different developmental stages. And the target genes were also predicted using miRBase, TargetScan7.2, and miRWalk, and finally the lncRNA-miRNA-mRNA regulatory network was constructed in Capra hircus. The results demonstrated that XLOC_015132 was widely expressed in different tissues of the goat, especially with significantly expression in the spleen, lung, kidney, brain and skin in Youzhou black goat than that of Youzhou local white goats(P<0.05). The qRT-PCR results of LncRNA XLOC_015132 in B16-F10 melanoma cells showed that the expression pattern of the lncRNA was increased during the B16 cell proliferation. The bioinformatics analysis of LncRNA XLOC_015132 and interacting molecules showed that LncRNA XLOC_015132/miR-150-5 p/CCND2 axis may play an important regulatory role in the melanogenesis by activating PI3 K-AKT and Wnt signaling pathways. Based on the results of this study, the expression of LncRNA XLOC_015132 may be positively correlated with melanin deposition.