石氏熏洗方药浴对膝骨关节炎家兔股动脉血流动力学的影响及温度探索

Effect of Shi’s Fumigation and Bath Formula medicinal bath on femoral artery hemodynamics and temperature exploration in rabbits with knee osteoarthritis

目的:研究石氏熏洗方药浴对膝骨关节炎(KOA)家兔股动脉血流动力学的影响并探索合适的治疗温度。方法:①60只新西兰兔随机分为高温组、中温组、低温组、假手术组和模型组,分别采用右膝关节注射木瓜蛋白酶和左旋半胱氨酸建立KOA模型。造模4周后,对各组家兔膝关节进行水浴温度梯度干预,共治疗4周。研究不同温度下纯净水浴对KOA家兔股动脉血流动力学的影响,探索得出水浴治疗的最佳干预温度。②将50只新西兰兔随机分为石氏熏洗方药浴组、纯净水浴组、扶他林治疗组和模型组,在上述水浴最佳干预温度基础上,进一步研究不同温度下药浴对KOA家兔股动脉血流动力学的影响。结果:①40℃时纯净水浴对股动脉内径、收缩期峰值流速、舒张期峰值流速和血管阻力指数等血流动力学指标的干预效果最优(P<0.05);②与其他温度药浴和扶他林治疗相比,40℃时石氏熏洗方药浴对KOA家兔血流动力学指标的改善最为明显(P<0.05)。结论:石氏熏洗方药浴可加快KOA家兔股动脉的血流速度,减轻血管阻力,改善局部血液循环;40℃为石氏熏洗方药浴治疗KOA的最佳温度。

Objective:To study the effect of Shi’s Fumigation and Bath Formula(SFBF)medicinal bath on the hemodynamics of femoral artery in rabbits with knee osteoarthritis(KOA)and explore the appropriate treatment temperature.Methods:①Sixty New Zealand rabbits were randomly divided into high-temperature group,medium-temperature group,low-temperature group,sham-operated group and model group.The KOA model was established by injecting papain and levocysteine into the right knee respectively.Four weeks after modeling,the knees of rabbits in each group were treated with pure water bath temperature gradient intervention for a total of 4 weeks to explore the appropriate treatment temperature.②Fifty rabbits were randomly divided into SFBF medicinal bath group,pure water bath group,votalin treatment group and model group to further study the effect of traditional Chinese medicine medicinal bath on the hemodynamics of femoral artery in KOA rabbits at different temperatures based on above optimal water bath intervention temperature.Results:①The intervention effect of pure water bath at 40℃was optimal(P<0.05)on hemodynamic indexes such as femoral artery internal diameter,systolic peak flow rate,diastolic peak flow rate and vascular resistance index.②Compared with medicinal bath at other temperatures and votalin treatment,the improvement of hemodynamic indexes on KOA rabbits of the SFBF medicinal bath at 40℃was most significant(P<0.05).Conclusion:SFBF medicinal bath can accelerate the blood flow rate of the femoral artery in KOA rabbits,reduce vascular resistance and improve local blood circulation.And 40℃is the optimal temperature for the treatment of KOA with the SFBF medicinal bath.