miR-637在桥本甲状腺炎合并甲状腺乳头状癌患者组织穿刺标本中的水平变化及对甲状腺癌细胞增殖、侵袭的影响

Changes in the level of miR-637 in tissue biopsy specimens of patients with Hashimoto′s thyroiditis complicated with papillary thyroid carcinoma and its effects on the proliferation and invasion of thyroid cancer cells

目的探讨miR-637在桥本甲状腺炎(HT)合并甲状腺乳头状癌(PTC)患者组织穿刺标本中的水平变化,以及其对甲状腺癌细胞增殖、迁移和侵袭的影响。方法收集2019年6月至2021年6月于该院治疗的92例HT患者的组织穿刺标本,其中单纯HT患者46例(单纯HT组),HT合并PTC患者46例(HT合并PTC组),采用实时定量荧光PCR(qPCR)对两组患者组织穿刺标本的miR-637水平进行检测。细胞学实验:将人甲状腺癌细胞TPC-1分为未转染质粒的对照组、转染阴性对照(NC)质粒的NC组和转染miR-637质粒的miR-637组。采用CCK8法检测各组细胞增殖能力,细胞划痕实验检测各组细胞迁移能力,Transwell实验检测各组细胞侵袭能力,Western blot检测迁移侵袭关键蛋白基质金属蛋白酶2(MMP2)和基质金属蛋白酶9(MMP9)蛋白的表达水平。结果与单纯HT组比较,HT合并PTC组组织穿刺标本miR-637水平降低,差异有统计学意义(P<0.01)。与对照组相比,miR-637组细胞增殖能力降低(P<0.05),迁移能力和侵袭能力显著下降(P<0.05)。Western blot显示:miR-637组MMP-2和MMP-9蛋白表达水平显著降低(P<0.05)。结论miR-637在PTC合并HT患者组织穿刺标本中水平降低,miR-637可以抑制人甲状腺癌细胞增殖能力、迁移能力和侵袭能力,其机制与抑制MMP2及MMP9蛋白表达相关。

Objective To investigate the level changes of miR-637 in tissue biopsy specimens of patients with Hashimoto′s thyroiditis(HT)combined with papillary thyroid carcinoma(PTC),and its effect on the proliferation,migration and invasion of thyroid cancer cells.Methods Tissue biopsy specimens of 92 HT patients treated in the hospital from June 2019 to June 2021 were collected,including 46 patients with only HT(simple HT group)and 46 patients with HT complicated with PTC(HT complicated with PTC group).Real-time quantitative PCR(qPCR)was used to detect the level of miR-637 in tissue biopsy specimens of the two groups of patients.Cytological experiment:human thyroid cancer cells TPC-1 were divided into control group which were without plasmid transfection,NC group transfected with negative control(NC)plasmid and miR-637 group transfected with miR-637 plasmid.The cell proliferation ability of each group was detected by CCK8 method,the migration ability of each group was detected by cell scratch assay,the invasion ability of each group was detected by Transwell assay,and the key proteins of migration and invasion,matrix metalloproteinase 2(MMP2)and matrix metalloproteinase 9(MMP2),were detected by Western blot.Results Compared with the simple HT group,the level of miR-637 in the tissue biopsy specimens of the HT complicated with PTC group decreased,and the difference was statistically significant(P<0.01).Compared with the control group,the proliferation ability of cells in the miR-637 group was reduced(P<0.05);the migration ability and invasion ability were significantly reduced(P<0.05).Western blot showed that the protein expression levels of MMP2 and MMP9 in the miR-637 group significantly decreased(P<0.05).Conclusion The level of miR-637 decreased in tissue biopsy specimens of patients with HT complicated with PTC.MiR-637 could inhibit the proliferation,migration and invasion of human thyroid cancer cells,and its mechanism was related to the inhibition of MMP2 and MMP9 protein expression.