lncRNA SNHG3在非小细胞肺癌中的表达及其临床意义

Expression and clinical significance of lncRNA SNHG3 in non-small cell lung cancer

目的:检测非小细胞肺癌(NSCLC)患者癌组织中长链非编码RNA(lncRNA)小核仁RNA宿主基因3(SNHG3)的表达水平,探讨其临床意义及与患者预后的关系。方法:选取2012年1月至2014年1月在本院确诊的94例NSCLC患者为研究对象,收集其癌组织及对应癌旁组织。实时荧光定量PCR(qRT-PCR)法检测患者癌组织、癌旁组织中lncRNA SNHG3表达水平,分析癌组织中lncRNA SNHG3与NSCLC患者临床特征的关系;随访5年生存情况,Kaplan-Meier生存曲线分析lncRNA SNHG3表达与生存时间的关系;Cox分析影响NSCLC患者预后的因素。结果:研究对象癌组织中lncRNA SNHG3表达水平高于癌旁组织,差异有统计学意义(P<0.05);癌组织中lncRNA SNHG3表达水平与年龄、肿瘤原发灶淋巴结转移(TNM)分期、分化程度、淋巴转移、吸烟史相关(P<0.05)。lncRNA SNHG3高表达组总生存时间短于lncRNA SNHG3低表达组,差异有统计学意义(P<0.05)。单因素Cox回归分析显示,lncRNA SNHG3表达水平、性别、TNM分期、分化程度、淋巴转移、吸烟史是NSCLC患者不良预后的危险因素;多因素Cox回归分析显示,lncRNA SNHG3表达水平、TNM分期、吸烟史是NSCLC患者不良预后的独立危险因素。结论:NSCLC患者癌组织中lncRNA SNHG3表达水平高于癌旁组织,与TNM分期密切关系,是不良预后的独立危险因素,可作为患者不良预后的预测指标。

Objective:To observe the expression level of long non-coding RNA(lncRNA)small nucleolar RNA host gene 3(SNHG3)in cancer tissues of patients with non-small cell lung cancer(NSCLC),and to explore its clinical significance and relationship with prognosis.Methods:Ninety-four NSCLC patients diagnosed in our hospital from January 2012 to January 2014 were regarded as the study objects,and the cancer tissue and the corresponding paracancerous tissues was included.The expression level of lncRNA SNHG3 was determined by real-time fluorescent quantitative PCR(qRT-PCR)method,the relationship between lncRNA SNHG3 and clinical characteristics of NSCLC was analyzed;after 5-year follow-up,the relationship between lncRNA SNHG3 and survival time was analyzed by Kaplan-Meier survival curve,and the factors influencing the prognosis of NSCLC patients were analyzed by Cox.Results:The expression level of lncRNA SNHG3 in cancer tissues of NSCLC patients is higher than that in paracancerous tissues(P<0.05);and the expression level of lncRNA SNHG3 in cancer tissues was correlated with age,tumor primary lymph node metastasis(TNM)stage,degree of differentiation,lymph node metastasis and smoking history(P<0.05).The total survival time of lncRNA SNHG3 high expression group was lower than that of lncRNA SNHG3 low expression group(P<0.05).Single factor Cox regression analysis showed that the expression level of lncRNA SNHG3,gender,TNM stage,degree of differentiation,lymph node metastasis and smoking history were risk factors for poor prognosis in NSCLC patients;and multivariate Cox regression analysis showed that lncRNA SNHG3 expression level,TNM stage and smoking history were independent risk factors for poor prognosis in NSCLC patients.Conclusion:The expression level of lncRNA SNHG3 in cancer tissues of NSCLC patients is higher than that in paracancerous tissues,which is closely related to TNM stage.It is an independent risk factor for poor prognosis and can be used as a predictor of poor prognosis.