二氢杨梅素及其酰化衍生物对肝细胞氧化损伤的保护作用

The protection effect of dihydromyricetin and its acylated derivatives on oxidative damage of hepatocytes

目的:考察二氢杨梅素(DHM)及其衍生物对肝细胞氧化损伤的保护作用。方法:建立L02细胞过氧化氢损伤模型,通过酶法酰化修饰DHM得到不同亲脂性的DHM衍生物,再通过测定活性氧(ROS)、乳酸脱氢酶(LDH)、丙二醛(MDA)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)水平、线粒体膜电位和Caspase 3水平评估DHM及其衍生物对肝损伤细胞的保护作用。结果:除3-O-月桂酰化二氢杨梅素外,其余衍生物处理后的肝损伤细胞中活性氧(ROS)、乳酸脱氢酶(LDH)的释放和丙二醛(MDA)水平显著降低,抗氧化酶系水平升高,线粒体膜电位升高,Caspase 3水平降低,其中3-O-辛酰化二氢杨梅素的保护效果最好。结论:中等链长二氢杨梅素衍生物有较好的护肝效果。

Objective:This study aimed to investigate the protective effect of dihydromyricetin(DHM)and its derivatives on oxidative damage of hepatocytes.Methods:The hydrogen peroxide damage model of L02 cells was established.DHM derivatives with different lipophilicity were obtained by enzymatic acylation of DHM.The levels of reactive oxygen species(ROS),lactate dehydrogenase(LDH),malondialdehyde(MDA),catalase(CAT),superoxide dismutase(SOD),glutathione peroxidase(GSH-PX),and mitochondrial membrane potential and Caspase 3 were measured to evaluate the protective effect of DHM and its derivatives on damaged hepatocytes.Results:Except for 3-O-lauroyl-DHM,the level of reactive oxygen species(ROS)the release of lactate dehydrogenase(LDH)and the level of malondialdehyde(MDA)and Caspase 3 in damaged hepatocytes treated with other derivatives decreased significantly,while the levels of antioxidant enzymes and the mitochondrial membrane potential increased.Of which,3-O-octanoyl-DHM showed the best protective effect.Conclusion:Dihydromyricetin derivatives with medium chain length have better liver protection effect.