细叶黄皮根总黄酮提取工艺优化及抑菌活性研究

Study on optimization of extraction technology and antibacterial activity of total flavonoids from the root of Clausena anisum-olens

目的:优化细叶黄皮根总黄酮的提取工艺,并测定不同产地细叶黄皮根总黄酮得率及其抑菌作用。方法:以细叶黄皮根为原料,采用超声波法提取总黄酮物质,通过单因素与正交试验,进行各产地细叶黄皮根总黄酮的提取及抑菌试验。结果:提取细叶黄皮根总黄酮最佳工艺为:温度70℃,超声功率50W,乙醇体积分数90%,料液比1:16(g/mL),提取时间40min,此条件下细叶黄皮根总黄酮得率为3.361%;崇左市区细叶黄皮根总黄酮得率最高达4.781%;细叶黄皮根总黄酮对绿脓杆菌、金色葡萄球菌、枯草杆菌、杀螟杆菌及大肠杆菌均有抑制作用,而且作用较强。结论:采用超声波法提取细叶黄皮根总黄酮较为适宜,不同产地细叶黄皮根总黄酮的得率差异较大,其总黄酮具有广谱的抑菌活性。

Objective:This study aimed to optimize the optimal extraction process of total flavonoids from the root of Clausena anisum-olens and determine the yield and antibacterial effect of total flavonoids from different producing areas.Methods:The total flavonoids were extracted by ultrasonic method from the roots of C.anisum-olens.Through single factor and orthogonal test,the effect of ultrasonic assisted extraction conditions on the extraction rate of total flavonoids from the root of C.anisum-olens was investigated,and the extraction and antibacterial test of total flavonoids from various producing areas were carried out.Results:The optimum extraction process of total flavonoids fromC.anisum-olens was as follows:temperature 70℃,ultrasonic power50 W,ethanol concentration 90%,solid-liquid ratio 1:16(g/mL),extraction time 40 min.Under the control of these conditions,the extraction rate of total flavonoids was 3.361%,and the yield of total flavonoids in the root bark of C.anisumolens in Chongzuo city was the highest,up to 4.781%;the total flavonoids of C.anisum-olens had strong inhibitory effects on Pseudomonas aeruginosa,Staphylococcus aureus,Bacillus subtilis,borer killing bacteria and Escherichia coli,indicating that it had broad-spectrum antibacterial activity.Conclusion:Ultrasonic method was suitable for the extraction of flavonoids from the root of C.anisum-olens.The yield of flavonoids from different producing areas varied greatly,and the total flavonoids had broad spectrum antibacterial activity.