超声辅助法提取酸枣仁中异牡荆素—吡喃葡萄糖苷工艺优化

Optimization of ultrasound-assisted extraction of isovitexinglucopyranoside from ziziphi spinosae semen seeds

目的:提高酸枣仁资源综合利用率。方法:以超声功率、超声时间、料液比、乙醇体积分数和超声温度为影响因素,以酸枣仁中异牡荆素—吡喃葡萄糖苷提取率为考察指标,运用响应面法优化异牡荆素—吡喃葡萄糖苷提取工艺参数。结果:异牡荆素—吡喃葡萄糖苷的最优提取工艺条件为超声功率405 W、超声时间60min、料液比1:16(g/mL)、乙醇体积分数75%,该条件下异牡荆素—吡喃葡萄糖苷提取率为0.705%,与预测值(0.719%)接近,表明异牡荆素—吡喃葡萄糖苷提取率的预测值与实际情况拟合良好。结论:超声辅助提取异牡荆素—吡喃葡萄糖苷的工艺稳定可靠,提取率高。

Objective:This study focused on improving comprehensive utilization rate of ziziphi spinosae semen resources.Methods:With the ultrasonic power,ultrasonic time,solid-liquid ratio,ethanol volume fraction and ultrasonic temperature as the influencing factors,and the extraction rate of isovitexin-glucopyranoside in ziziphi spinosae semen as the index,the extraction process parameters of isovitexin-glucopyranoside were optimized by response surface methodology.Results:The optimal extraction conditions of isovitexin-glucopyranoside were ultrasonic power 405 W,ultrasonic time 60 min,solid-liquid ratio1:16(g/mL)and ethanol volume fraction 75%.Under the control of these conditions,the extraction rate of isovitexin-glucopyranoside was 0.705%,which was close to the predicted value(0.719%),indicating that the predicted value of the extraction rate of isovitexin-glucopyranoside fit well with the actual situation.Conclusion:The ultrasonic assisted extraction of isovitexin-glucopyranoside is stable and reliable with high extraction rate.