DPPH体系优化及无花果黄酮清除自由基性能研究

Optimization of DPPH Reaction System and Free Radical Scavenging Performance of Total Ficus carica Linn.Flavonoids

目的:通过对避光情况、实验时间长短、实验温度的考察,得到最适的DPPH反应体系,同时研究无花果中黄酮对DPPH自由基的清除能力。方法:利用超声-微波辅助法提取无花果中的总黄酮,通过紫外分光光度计考察DPPH-CH_(2)CH_(3)OH体系的重复性。用DPPH法进行清除自由基性能研究,对比3种品种(青皮、波姬红、布兰瑞克)的抗氧化能力。结果:无花果提取物清除DPPH反应体系的最优条件为避光条件下,实验温度为25℃时反应30 min。在0.2~1.0 mg/mL,随浓度的增大,青皮、波姬红、布兰瑞克黄酮提取物清除自由基的性能越好,其中布兰瑞克黄酮提取物的清除自由基能力最强。结论:无花果中的黄酮化合物具有良好的清除自由基的能力。

Objective:The optimal DPPH reaction system was obtained by investigating the conditions of light avoidance,the length of experimental time,and the experimental temperature.At the same time,the free radical scavenging ability of flavonoids in Ficus carica Linn.to DPPH was studied.Methods:The total flavonoids in Ficus carica Linn.were extracted by ultrasound-microwave assisted Methods.The reproducibility of DPPH-CH_(2)CH_(3)OH was investigated by UV spectrophotometer.Free radical scavenging performance studies were carried out by the DPPH Methods,and the antioxidant capacity of three varieties(Qingpi,Bojihong and Braunschweig)was compared.Results:The optimum conditions for the removal of DPPH by Ficus carica Linn.extract were as follows:in dark conditions,the experiment temperature was 25℃ and the reaction time was 30 min.At the concentration of 0.2~1.0 mg/mL,with the increase of concentration,the free radical scavenging performance of Qingpi,Bojihong and Braunschweig has the strongest free radical scavenging ability.Conclusion:The flavonoids in Ficus carica Linn.have a good ability to scavenge free radicals.