胰高血糖素样肽-1受体rs3765467基因多态性与冠心病的相关性研究

Correlation Between Glucagon-like Peptide-1 Receptor rs3765467 Gene Polymorphism and Coronary Heart Disease

目的探究胰高血糖素样肽-1受体(glucagonlike peptide-1 receptor,GLP-1R)rs3765467位点基因多态性与冠心病的相关性研究。方法采用病例对照研究,选取行选择性冠状动脉造影检查的病人280例,根据造影检查结果分为健康对照组148例和病例组(冠心病组)132例。研究比较两组一般资料、高血压病史、总胆固醇(total cholesterol,TC)、甘油三酯(triacylglycerol,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterin,LDL-C)、载脂蛋白A1(apolipoprotein A1,ApoA1)、载脂蛋白B(apolipoprotein B,ApoB)、GLP-1R rs3765467位点基因型及等位基因分布,比较病例组GLP-1R基因rs3765467位点不同基因型血脂指标,研究GLP-1R基因rs3765467位点与冠心病发病风险的关系。结果病例组中年龄、性别比例、吸烟比史、BMI、TC、LDL-C、ApoA1、既往高血压病史差异具有统计学意义(P<0.05)。GLP-1R基因rs3765467位点具有多态性,其位点有TT、CT和CC 3种基因型,其分布均符合H-W平衡。在病例组GLP-1R基因rs3765467位点T等位基因突变者血脂甘油三酯(TG)水平明显高于C等位基因人群,差异有统计学意义(P<0.05)。其中女性携带TT+TC基因型比携带CC基因型具有更高水平的TG(P<0.05)。排除冠心病传统危险发病因素后,采用Logistic回归分析,与携带CT/CC基因型相比,携带TT基因型的人群患冠心病的风险增加4.681倍(OR=4.681,95%CI:1.017~21.534,P=0.047),差异有统计学意义(P<0.05)。结论GLP-1R基因rs3765467位点多态性影响血脂水平,可能与冠心病发病相关,TT基因型增加了冠心病的患病风险,T等位基因为冠心病的风险基因。

Objective To investigate the correlation between glucagonlike peptide-1 receptor(GLP-1R)rs3765467 gene polymorphism and coronary heart disease.Methods In a case-control study,280 patients who underwent selective coronary angiography were selected and divided into healthy control group(148 cases)and case group(132 cases)according to the results of angiography.The study compared the general data,history of hypertension,total cholesterol(TC),triacylglycerol(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),apolipoprotein A1(ApoA1),apolipoprotein B(ApoB),and genotype and allele distribution of GLP-1R rs3765467 locus between the two groups.The relationship between the rs3765467 locus of GLP-1R gene and the risk of coronary heart disease was studied by comparing the lipid indexes of different genotypes of rs3765467 locus of GLP-1R gene in the case group.Results There were statistically significant differences in age,sex ratio,smoking history,BMI,TC,LDL-C,ApoA1 and past hypertension history in the case group(P<0.05).The rs3765467 of GLP-1R gene was polymorphic,and there were three genotypes of TT,CT and CC,and their distribution was in accordance with H-W equilibrium.The TG level of T allele mutation at rs3765467 of GLP-1R gene in case group was significantly higher than that in C allele group,with statistical significance(P<0.05).Women with TT+TC genotype had higher TG level than those with CC genotype(P<0.05).After excluding the traditional risk factors for CHD,Logistic regression analysis showed that the risk of CHD was 4.681 times higher in the TT genotype than in the CT/CC genotype(OR=4.681,95%CI:1.017~21.534,P=0.047),the difference was statistically significant(P<0.05).Conclusion Glp-1R gene rs3765467 polymorphism affects blood lipid levels and may be associated with the incidence of coronary heart disease.TT genotype increases the risk of coronary heart disease,and T allele is the risk gene of coronary heart disease.