青花菜细胞质雄性不育相关LncRNAs的鉴定与表达分析

Identification and Expression Analysis of Cytoplasmic Male Sterile Related LncRNAs in Broccoli

细胞质雄性不育系的应用可有效提高杂交种质量。该研究利用illumina测序技术鉴定青花菜细胞质雄性不育相关LncRNAs,对其靶基因和表达特征进行分析,并随机选取16个LncRNAs用qRT-PCR技术检测其表达特征,为进一步阐明LncRNA参与青花菜雄性不育发生机制提供新的路径。结果表明:(1)鉴定获得青花菜雄性不育相关LncRNAs共4326个,其中37个LncRNA在不育系及其保持系中差异表达。(2)差异LncRNAs可预测得到370个cis靶基因,这些靶基因部分为雄性不育相关的转录因子和生物蛋白。(3)XLOC_006651、XLOC_016660、XLOC_003494和XLOC_013121在不育系和保持系花蕾发育早期表达量较高,之后随着花蕾的发育,表达量逐渐下降;XLOC_021769和XLOC_038964呈先降后升的表达模式,且XLOC_038964和XLOC_012613在花蕾不同发育阶段不育系的表达量均高于保持系。(4)16个LncRNA均可在花梗、花萼、花瓣、雄蕊及雌蕊中表达,且XLOC_038964、XLOC_011575、XLOC_013157、XLOC_039677、XLOC_037356、XLOC_034182和XLOC_017574呈现出相似的表达模式,在雄蕊和花梗中表达量较低,花萼和花瓣中较高,雌蕊中表达量最高。

The application of cytoplasmic male sterile(CMS)line can effectively improve hybrids quality.In this study,the LncRNAs related to cytoplasmic male sterility in broccoli were identified by Illumina sequencing,and their target genes and expression characteristics were also analyzed.Meanwhile,the expression patterns of 16 randomly selected LncRNAs were detected by qRT-PCR technology,which could provide new insights for further clarifying the mechanism of occurrence of male sterility involved by LncRNAs in broccoli.The results show that:(1)4326 LncRNAs related to male sterility were identified in broccoli.Among them,37 LncRNA were differentially expressed between male sterility line and its maintainer.(2)370 cis-target genes of these differentially expressed LncRNA were predicted,many of these target genes were transcription factors and bioproteins related to CMS.(3)XLOC_006651,XLOC_016660,XLOC_003494 and XLOC_013121 were highly expressed in early development stage of bud in male sterile line and its maintainer line,and then decreased gradually with development of bud;XLOC_021769 and XLOC_038964 showed a falling-rising expression pattern.And their expression were higher in male sterile line than that in maintainer line at different development stages of bud.(4)All sixteen LncRNA could be detected in pedicel,calyx,petal,stamen and pistil.XLOC_038964,XLOC_011575,XLOC_013157,XLOC_039677,XLOC_037356,XLOC_034182 and XLOC_017574 showed similar expression patterns,with low expression in stamen and pedicel,higher expression in calyx and petal,and the highest expression in pistil.