低强度微波对急性脊髓损伤大鼠体感诱发电位和运动诱发电位的影响

Effects of Low Intensity Microwave on Somatosensory Evoked Potentials and Motor Evoked Potentials in Rats with Acute Spinal Cord Injury

目的:观察低强度微波对急性脊髓损伤模型大鼠体感诱发电位(SEPs)和运动诱发电位(MEPs)的影响。方法:72只成年雌性SD大鼠随机分为Sham组、SCI组和MW组,每组24只。Sham组仅行椎板切除术,SCI组和MW组采用改良Allen’s法制备脊髓损伤模型。造模后MW组给予低强度微波治疗,SCI组和Sham组不给予任何治疗。在术后1d、7d、14d和21d对大鼠进行BBB评分、SEPs和MEPs评定。结果:BBB评分显示,MW组大鼠在术后7d和14d时与SCI组相比变化不明显(P>0.05),术后21d时MW组优于SCI组(P<0.01);SEPs和MEPs数值显示,MW组与SCI组在术后7d时神经功能变化不明显(P>0.05),而在术后14d和21d时均明显改善(P<0.05)。结论:低强度微波能促进急性脊髓损伤大鼠神经功能恢复,微波电磁场对脊髓损伤修复具有一定的应用价值。

Objective:To observe the effects of low intensity microwave on somatosensory evoked potentials(SEPs)and motor evoked potentials(MEPs)in rats with acute spinal cord injury.Methods:A total of 72 adult female SD rats were randomly divided into Sham group,SCI group and MW group,there were 24 rats in each group.The Sham group only received laminectomy to expore the dura mater.The models of spinal cord injury were established by a modified Allen’s method in SCI and MW groups.The rats in MW group received low intensity microwave irradiation after surgery,and the rats in the other groups weren’t given any treatment after surgery.The recovery of hindlimb function of the rats in three groups was evaluated by Basso-Beattie-Bresnahan(BBB)score at 1,7,14 and 21 days after surgery.SEPs and MEPs were also performed to detect the neural conduction of the rats in various groups at the same time.Results:There were no statistics significant increase in the BBB scores between MW group and SCI group at 7 days and 14 days after surgery(P>0.05),while a significant increase in the BBB scores was observed in MW group compared to those in SCI group at 21 days after surgery(P<0.05).The results of SEPs and MEPs showed that the recovery of spinal cord function in MW group was improved obviously compared with SCI group at 14 days and 21 days after surgery(P<0.05),while a improvement in MW group was no statistics significant improved at 7 days after surgery(P>0.05).Conclusion:Low intensity microwave irradiation could promote the recovery of spinal cord functional in rats with acute spinal cord injury.The microwave electromagnetic field might have certain application value in the repair of spinal cord injury.