lncRNA TGFB2-AS1在子痫前期胎盘中表达及与胎盘螺旋动脉重铸的关系

Expression of lncRNA TGFB2-AS1 in the placenta of preeclampsia and its relationship with placental spiral artery recasting

目的探讨子痫前期胎盘中长链非编码RNA转化生长因子β2-反义RNA1(lncRNA TGFB2-AS1)表达水平与胎盘螺旋动脉重铸的关系。方法选取2019年10月至2021年6月于枣庄市妇幼保健院住院并行剖宫产术分娩的108例重度子痫前期孕妇为研究对象,并将其分为晚发子痫前期组(晚发型重度子痫前期孕妇,56例)和早发子痫前期组(早发型重度子痫前期孕妇,52例);同期选取58例正常妊娠孕妇为正常妊娠组。收集孕妇年龄、收缩压、舒张压等一般资料;采用实时荧光定量PCR(qRT-PCR)法检测胎盘组织lncRNA TGFB2-AS1表达水平,扫描电子显微镜下测量螺旋动脉管腔面积和管壁厚度;采用Pearson相关分析法分析早发型和晚发型重度子痫前期孕妇胎盘组织lncRNA TGFB2-AS1水平与螺旋动脉管壁厚度、管腔面积的相关性。结果早发子痫前期组管壁厚度[(119.69±8.31)μm]、收缩压[(162.86±4.94)mmHg]、舒张压[(103.09±2.35)mmHg]、24 h尿蛋白[(2.17±0.31)g/24 h]高于晚发子痫前期组[(101.04±5.78)μm、(146.95±6.43)mmHg、(92.13±4.74)mmHg、(1.62±0.23)g/24 h]和正常妊娠组[(99.82±5.56)μm、(116.42±9.31)mmHg、(74.25±6.74)mmHg、(0.06±0.02)g/24 h];胎盘组织lncRNA TGFB2-AS1水平(0.62±0.16)及管腔面积[(133.74±20.16)μm^(2)]、分娩孕周[(32.15±1.74)周]、新生儿体质量[(2.25±0.26)g]低于晚发子痫前期组[(0.99±0.21)、(185.49±22.75)μm^(2)、(36.14±1.59)周、(3.37±0.32)g]和正常妊娠组[(1.02±0.23)、(186.42±23.71)μm^(2)、(38.19±1.56)周、(3.42±0.37)g](P<0.05)。晚发子痫前期组收缩压、舒张压、24 h尿蛋白高于正常妊娠组,分娩孕周低于正常妊娠组(P<0.05)。早发型重度子痫前期孕妇胎盘组织lncRNA TGFB2-AS1与管腔面积呈正相关,与管壁厚度呈负相关(P<0.05);晚发型重度子痫前期孕妇胎盘组织lncRNA TGFB2-AS1与管腔面积、管壁厚度均无相关性(P>0.05)。结论早发型重度子痫前期孕妇胎盘组织lncRNA TGFB2-AS1异常低表达,可能与胎盘螺旋动脉重铸不足有关。

Objective To investigate the relationship between the expression level of long non-coding RNA transforming growth factorβ2-antisense RNA1(lncRNA TGFB2-AS1)and placental spiral artery recasting in the placenta of preeclampsia.Methods A total of 108 pregnant women with severe preeclampsia who were hospitalized in Zaozhuang Maternal and Child Health Hospital and delivered by cesarean section from Oct.2019 to Jun.2021 were selected as the research objects,and they were divided into the late-onset preeclampsia group(late-onset severe preeclampsia pregnant women,56 cases)and early-onset preeclampsia group(early-onset severe preeclampsia pregnant women,52 cases);at the same time,58 normal pregnant women were selected as the normal pregnancy group.The general data of pregnant women were collected,such as age,systolic blood pressure and diastolic blood pressure.Real-time fluorescent quantitative PCR(qRT-PCR)method was used to detect the expression level of lncRNA TGFB2-AS1 in placental tissues,a scanning electron microscope was used to measure the lumen area and wall thickness of spiral arteries.Pearson correlation analysis method was used to analyze the correlation between the level of lncRNA TGFB2-AS1 in the placenta tissue and the thickness of the spiral artery wall and the area of the lumen of pregnant women with early-onset and late-onset severe preeclampsia.Results The tube wall thickness[(119.69±8.31)μm],systolic blood pressure[(162.86±4.94)mmHg],diastolic blood pressure[(103.09±2.35)mmHg],and 24-hour urine protein[(2.17±0.31)g/24 h]in the early preeclampsia group were higher than those in the late preeclampsia group[(101.04±5.78)μm,(146.95±6.43)mmHg,(92.13±4.74)mmHg,(1.62±0.23)g/24 h]and the normal pregnancy group[(99.82±5.56)μm,(116.42±9.31)mmHg,(74.25±6.74)mmHg,(0.06±0.02)g/24 h],the placental tissue lncRNA TGFB2-AS1 level(0.62±0.16),lumen area[(133.74±20.16)μm^(2)],gestational week of delivery[(32.15±1.74)weeks],weight of the newborns[(2.25±0.26)g]were lower than those in the late-onset preeclampsia group[(0.99±0.21),(185.49±22.75)μm^(2),(36.14±1.59)weeks,(3.37±0.32)g]and the normal pregnancy group[(1.02±0.23),(186.42±23.71)μm^(2),(38.19±1.56)weeks,(3.42±0.37)g](P<0.05).The systolic blood pressure,diastolic blood pressure,and 24-hour urine protein in the late preeclampsia group were higher than those in the normal pregnancy group,gestational week of delivery was lower than the normal pregnancy group(P<0.05).Placental tissue lncRNA TGFB2-AS1 of pregnant women with early-onset severe preeclampsia was positively correlated with the lumen area,and negatively correlated with the thickness of the tube wall(P<0.05).There was no correlation between lncRNA TGFB2-AS1 and the lumen area and wall thickness in the placental tissue of pregnant women with late-onset severe preeclampsia(P>0.05).Conclusion The lncRNA TGFB2-AS1 expression in the placenta tissue of pregnant women with early-onset severe preeclampsia is abnormally low,which may be related to the insufficient recasting of the placental spiral artery.