普通烟草热激转录因子家族的全基因组鉴定与分析

Genome-wide Identification and Systemic Analysis of the Hsf Gene Family in Nicotiana tabacum L.

热激转录因子(heat shock transcription factor, Hsf)家族成员广泛参与植物生长发育和非生物胁迫的调控。本研究应用生物信息学和比较基因组学方法从普通烟草中鉴定了49个Hsf家族成员,发现这些Hsf家族成员均具有较为保守的寡聚化结构域。通过系统进化分析可将烟草和拟南芥的Hsf家族成员划分成3个分支与14个亚家族。共线性分析显示烟草Hsf基因与双子叶植物(拟南芥,番茄和葡萄)Hsf基因组成共线基因对的数量要大于与单子叶植物(水稻和玉米)Hsf基因组成共线基因对的数量。全基因组复制预测共有12个NtHsf基因可组成7条复制基因对。启动子分析发现NtHsf基因启动子区域存在着多个与非生物胁迫相关的顺式作用元件。表达模式分析证实NtHsf家族成员在不同组织的表达具有差异,并且大多数NtHsf家族成员可响应热和干旱胁迫。研究结果可为烟草Hsf转录因子家族成员的功能研究奠定生物信息学基础。

Heat shock transcription factor(Hsf) family members are widely involved in the regulation of plant development and abiotic stress responses. In the current study, 49 Hsf members were identified from the tobacco genome by bioinformatics and comparative genomics methods, all of which contained conserved oligomerization domains. The Hsf members from tobacco and Arabidopsis were categorized into 3 clades and 14 subfamilies according to the phylogenetic analysis. The number of collinear gene pairs between NtHsf and Hsf genes from dicots(Arabidopsis thaliana, tomato and grape) was more than the collinear gene pairs with the Hsf genes from monocots(rice and maize). Besides, a total of 12 NtHsf genes were predicted to have arisen from duplication events. Promoter analysis showed that there were a variety of cis-elements related to abiotic stress responses on the NtHsf promoters. The various expression patterns of NtHsf in different tissues were detected, and most NtHsf genes were induced by heat and drought stress. The results presented by this study might provide insights for the further studies on NtHsf members.