肾上腺素联合冰水浴制备气滞血瘀证大鼠中医症状及生物学指标的动态观察

Dynamic Observation on TCM Symptoms and Biological Indexes of Rats with Qi Stagnation and Blood Stasis Syndrome Prepared by Adrenaline Combined with Ice Water Bath

目的:通过动态观察大鼠中医症状表现及相关生物学指标评价肾上腺素联合冰水浴制备的气滞血瘀证大鼠模型。方法:将SD大鼠随机分为正常组、气滞血瘀证12 h、24 h、48 h、96 h组,采用皮下注射肾上腺素联合冰水浴的方法建立气滞血瘀证模型,于造模后4个时间点动态观察大鼠中医症状及生物学指标变化,检测造模后24 h大鼠血管和心肌损伤情况。结果:与正常组比较,气滞血瘀证12 h、24 h、48 h、96 h组大鼠均表现出血瘀证症状,证候积分明显升高,舌、足、尾的红色(red,R)分量值明显下降(P<0.05,P<0.01),心电图参数明显异常(P<0.05,P<0.01),血液流变学指标显著升高(P<0.05,P<0.01),血脂4项指标异常(P<0.05,P<0.01)。此外,气滞血瘀证24 h组较正常组的血清一氧化氮(nitric oxide,NO)含量显著降低,内皮素(endothelin-1,ET-1)含量显著升高,谷草转氨酶(aspertate aminotransferase,AST)、乳酸脱氢酶(lactic dehydrogenase,LDH)、肌酸激酶同工酶(creatine Kinase-MB,CK-MB)水平明显升高(P<0.05,P<0.01);腹主动脉及心肌组织的HE染色结果显示血管和心肌明显受损。结论:肾上腺素联合冰水浴造模后96 h仍可维持血瘀症状及气滞血瘀证的大部分病理特征,气滞血瘀证的发生发展可能与其血管内皮及心肌组织损伤密切相关。

Objective:To evaluate rats model with Qi stagnation and blood stasis syndrome prepared by adrenaline combined with ice water bath by dynamic observation on rat TCM symptoms and related biological indicators.Methods:Rats were randomly divided into normal groups and Qi stagnation and blood stasis syndrome 12 h,24 h,48 h,and 96 h group.A model of Qi stagnation and blood stasis syndrome was established by subcutaneous injection of adrenaline combined with ice bath.The rats’TCM symptoms and biological indicators were dynamically observed at 4 time points after modeling,and their vascular and myocardial damage 24 h after modeling were detected.Results:Compared with the normal group,the rats at 12 h,24 h,48 h,and 96 h after modeling all showed obvious TCM symptoms,the syndrome score was significantly increased,and the R(red)component values of tongue,foot,and tail were significantly decreased(P<0.05,P<0.01).At the same time,the ECG parameters were obviously abnormal(P<0.05,P<0.01);the blood rheology became worse(P<0.05,P<0.01);the blood lipids were abnormally increased(P<0.05,P<0.01).In addition,the NO content of rats with Qi stagnation and blood stasis syndrome was significantly reduced,and the ET-1 content markedly increased,while the levels of AST,LDH and CK-MB increased significantly(P<0.05,P<0.01).HE staining also showed that the blood vessels and myocardium were obviously damaged in the rats of 24 h group.Conclusion:The rats with Qi stagnation and blood stasis syndrome prepared by adrenaline combined with ice water bath met the TCM and clinical characteristics of Qi stagnation and blood stasis syndrome 96 hours after modeling.Moreover,the occurrence and development of Qi stagnation and blood stasis syndrome may be closely related to the damage of vascular endothelium and myocardial tissue.