丝素蛋白水凝胶支架的制备及其性能

Preparation and property of silk fibroin based hydrogel scaffolds

为探究丝素蛋白水凝胶材料的制备条件及其生物相容性,以二肉豆蔻酰磷脂酰甘油(DMPG)和脱胶丝素蛋白(SF)构成可控水凝胶体系,通过磷脂和SF链之间的静电和疏水作用,使SF链向β折叠结构转变获得SF水凝胶支架,分析了其细胞增殖率、细胞分化率、动物体内支架细胞肌肉生长速度和降解速度等。结果表明:DMPG浓度越高,SF水凝胶支架凝胶时间越短,添加15 mmol/L的DMPG使SF水凝胶支架的凝胶时间由原来的7 d缩短至10 min;DMPG诱导的SF水凝胶支架无细胞毒性,其中DMPG浓度为10 mmol/L时细胞增殖率最高,将该水凝胶注射入湖羊耳部皮下,其中载有湖羊肌肉卫星细胞的SF水凝胶支架在湖羊耳部生长出肌肉;SF水凝胶支架具有优异的生物相容性,随着其在动物体内时间的增加,支架逐渐降解消失,暴露出其中的肌肉组织。

In order to explore the preparation conditions and biocompatibility of silk fibroin hydrogel materials,a controllable hydrogel system consisting of two myristic phosphatidylglycerol(DMPG)and degumming silk fibroin(SF)was prepared.The SF chain was oriented by electrostatic and hydrophobic interactions between phospholipids and SF chainsβ.The SF hydrogel scaffolds were obtained by folding structure transformation.The cell proliferation rate,cell differentiation rate,the growth rate of animal scaffold cells and animal scaffold degradation rate were compared by different molar concentration of scaffolds.The results showed that the higher the concentration of DMPG was,the shorter the preparation time of SF hydrogel scaffolds.The 15 mmol/L DMPG shortened the preparation time of SF hydrogel scaffolds from 7 d to 10 min.DMPG induced SF hydrogel demonstrated no cytotoxicity,and when DMPG was 10 mmol/L the cell proliferation rate was the highest.SF hydrogel scaffold with DMPG concentration of 10 mmol/L was injected into the subcutaneous tissue of the Hu sheep′s ear.The SF hydrogel scaffold containing Hu sheep muscle satellite cells grew muscle in the animal body,and no muscle could be produced by simply injecting scaffolds or cells.SF hydrogel scaffolds possess excellent biocompatibility,and with the increase of SF hydrogel scaffolds in animal,the scaffold gradually degraded and disappeared,exposing the muscle tissue.